Co-expression of surfactant protein A and chicken lung lectin in chicken respiratory system. (June 2020)

• Record Type: Journal Article
• Authors: Liu, Hongmei; Huang, Xueting; Xiong, Haifeng; Liu, Miaomiao; Hu, Dongmei; Wei, Changqing; Wang, Guijun; Qi, Kezong
• Is Part Of: Molecular immunology. Volume 122(2020:Jun.)
• Page Start: 49
• Page End: 53
• Publication Date: 2020-06
• Subjects: Chicken surfactant protein A -- Chicken lung lectin -- Expression -- Co-localization -- Respiratory system; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2020.03.018
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: cSP-A and cLL mRNA were highly expressed in lungs compared to other tissues. mpared to other tissues. 2. Co-expression of cSP-A and cLL were mainly detected in epithelial cells of the chicken respiratory system. In contrast to cLL, which seems to have a wide distribution, cSP-A is mainly restricted to mucosal surfaces of the chicken respiratory tract. Abstract: Chicken surfactant protein A (cSP-A) and chicken lung lectin (cLL) are C-type lectins that play important roles in pulmonary host defense responses. Herein, we explored the localization of cSP-A and cLL in the chicken respiratory system. Six tissues from 30-days-old SPF chickens were used to quantify the expression of cSP-A and cLL using the quantitative real-time reverse transcriptional polymerase chain reaction (qRT-PCR) and fluorescence multiplex immunohistochemistry staining (fluorescence mIHC staining). Results showed that cSP-A and cLL mRNA were highly expressed in lungs compared to other tissues. cSP-A mRNA expression levels in all tissues were higher compared with cLL expression levels as analyzed using qRT-PCR. Fluorescence mIHC co-expression of cSP-A and cLL were mainly detected in lung parabronchial epithelia, and mucosal epithelia of larynx, trachea, syrinx, bronchus and air sac, with cSP-A showing a stronger positive staining compared with cLL. cLL is expressed on both mucosal surfaces, some individual lung epithelial cells and cartilage cells, while cSP-A is mainly restricted to mucosal surfaces of the respiratory tract. These histological findings may be useful for understanding the biological significance of this pulmonary lectins in future studies.