Effects of temperature changes on life parameters, oxidative stress, and antioxidant defense system in the monogonont marine rotifer Brachionus plicatilis. (June 2020)

• Record Type: Journal Article
• Authors: Han, Jeonghoon; Lee, Jin-Sol; Park, Jun Chul; Hagiwara, Atsushi; Lee, Kyun-Woo; Lee, Jae-Seong
• Is Part Of: Marine pollution bulletin. Volume 155(2020)
• Publication Date: 2020-06
• Subjects: Population growth -- Lifespan -- Reactive oxygen species -- Glutathione-S transferase -- Catalase -- Superoxide dismutase; Marine pollution -- Periodicals; Marine Biology -- Periodicals; Water Pollution -- Periodicals; Mer -- Pollution -- Périodiques; Publications périodiques; Pollution des mers; Lutte antipollution; Electronic journals; 363.7394
• DOI: 10.1016/j.marpolbul.2020.111062
• Languages: English
• ISSNs: 0025-326X

Abstract

Abstract: Global warming is a big concern for all organisms and many efforts have been made to reveal the potential effects of temperature elevation on aquatic organisms. However, limited studies on molecular mechanistic approaches on physiological effects due to temperature changes are available. Here, we investigated the effects of temperature changes on life parameters (e.g., population growth [total number of rotifers], and lifespan), oxidative stress levels and antioxidant activities (e.g., glutathione S -transferase [GST], catalase [CAT], superoxide dismutase [SOD]) with expression levels in the monogonont marine rotifer Brachionus plicatilis . The changes in temperatures led to significant reduction ( P < 0.05) in lifespan, possibly due to significant decrease ( P < 0.05) in antioxidant activities, reducing the potential to cope with significant elevation in the temperature-induced oxidative stress in B. plicatilis . To further assess the actual induction and clearance of reactive oxygen species (ROS), N -acetyl-L-cysteine was used to examine whether the temperature-induced oxidative stress could be successfully scavenged. Furthermore, expression patterns of the antioxidant-related genes ( GST s, SOD s, and CAT s) were down- or upregulated ( P < 0.05) in response to different temperatures in B. plicatilis . Overall, these findings indicate that ROS-mediated oxidative stress led to cellular damage and antioxidant defense system, resulting in deleterious effects on life parameters in rotifer. Highlights: The changes in temperatures led to significant reduction in lifespan. Significant reduction in lifespan would be due to significant decrease in antioxidant activities. N -acetyl-L-cysteine was successfully scavenged temperature-induced oxidative stress. GSTs, SODs, and CATs were down- or upregulated in response to different temperatures.