Black carp TRAFD1 restrains MAVS-mediated antiviral signaling during the innate immune activation. Issue 103 (August 2020)

• Record Type: Journal Article
• Authors: Yan, Jun; Zhang, Yinyin; Tan, Yaqi; Dai, Yuhan; Wei, Jing; Cao, Yingyi; Feng, Hao
• Is Part Of: Fish & shellfish immunology. Issue 103(2020)
• Page Start: 66
• Page End: 72
• Publication Date: 2020-08
• Subjects: TRAFD1 -- MAVS -- Interferon -- GCRV -- SVCV; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2020.04.045
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: TRAFD1 negatively regulates TLR and RLR signaling in human and mammal; however, its role in teleost fish remains unknown. In this paper, the TRAFD1 homologue has been cloned and characterized from black carp ( Mylopharyngodon piceus ). Black carp TRAFD1 (bcTRAFD1) consists of 567 amino acids and shows low similarity to that of mammalian TRAFD1, which has been identified as a cytosolic protein through immunofluorescence staining. When co-expressed with bcTRAFD1, the IFN promoter-inducing ability of black carp MAVS (bcMAVS) was obviously dampened in the luciferase reporter assay. Accordingly, bcMAVS-mediated antiviral activity against grass carp reovirus (GCRV) and spring viremia of carp virus (SVCV) was potently repressed by bcTRAFD1 in plaque assay. And the co-immunoprecipitation assay between bcTRAFD1 and bcMAVS has identified the association between these two molecules. Thus, our data supports the conclusion that bcTRAFD1 interacts with bcMAVS and negatively regulates bcMAVS-mediated antiviral signaling during the innate immune activation, which sheds a light on the regulation of MAVS in teleost. Highlights: bcTRAFD1 dampens the IFN promoter-inducing ability of bcMAVS. bcMAVS-mediated antiviral activity was potently repressed by bcTRAFD1. bcTRAFD1 interacts with bcMAVS.