Cellular localization, cloning and expression of Leishmania braziliensis Phospholipase A1. (April 2020)

• Record Type: Journal Article
• Authors: Bott, Emanuel; López, María Gabriela; Lammel, Estela María; Carfagna, Ivanna Emilce; Durante de Isola, Elvira Luisa; Ruybal, Paula; Taboga, Oscar; Gimenez, Guadalupe; Belaunzarán, María Laura
• Is Part Of: Microbial pathogenesis. Volume 141(2020)
• Publication Date: 2020-04
• Subjects: Leishmania braziliensis -- Phospholipase A1 -- Phosphatydilcholine -- Baculovirus expression system -- Phylogenetic analysis; BEVS baculovirus expression vector system -- LbPLA1 recombinant L. braziliensis Phospholipase A1 -- LPC lysophosphatidylcholine -- PC phosphatidylcholine -- PLA1 Phospholipase A1; Pathogenic microorganisms -- Periodicals; Pathology, Molecular -- Periodicals; Communicable Diseases -- microbiology -- Periodicals; Communicable Diseases -- parasitology -- Periodicals; Micro-organismes pathogènes -- Périodiques; Pathologie moléculaire -- Périodiques; Electronic journals; 616.9041
• DOI: 10.1016/j.micpath.2020.104010
• Languages: English
• ISSNs: 0882-4010

Abstract

Abstract: Leishmaniasis is caused by several species of protozoan parasites of the genus Leishmania and represents an important global health problem. Leishmania braziliensis in particular is responsible of cutaneous and mucocutaneous forms of this parasitosis, with prevalence in Latin America. In the present work, we describe in L. braziliensis promastigotes and amastigotes the presence of a Phospholipase A1 (PLA 1 ) activity, an enzyme that catalyses extensive deacylation of phospholipids like phosphatidylcholine. In order to deepen the knowledge about L. braziliensis PLA1, the cloning and expression of the gene that codifies for this enzyme was carried out in a baculovirus expression system with the obtaintion of a purified recombinant protein that displayed PLA1 activity. Given that this is the first molecular and functional protein characterization of a PLA1 in the Leishmania genus, we also performed a phylogenetic analysis of this gene throughout 12 species whose genome sequences were available. The results presented here will contribute to increase the knowledge about trypanosome phospholipases, which could be novel and valuable as potential targets to fight neglected diseases like Leishmaniasis. Graphical abstract: Image 1 Highlights: Leishmania braziliensis promastigotes & amastigotes possess Phospholipase A1 activity. L. braziliensis Phospholipase A1 gene was cloned and expressed in Sf9 insect cells. Purified recombinant Phospholipase A1 displayed enzyme activity. Phylogenetic analysis of Phospholipase A1 gene was done with 12 Leishmania species.