A comparative plastomics approach reveals available molecular markers for the phylogeographic study of Dendrobium huoshanense, an endangered orchid with extremely small populations. Issue 12 (30th April 2020)

• Record Type: Journal Article
• Authors: Niu, Zhitao; Hou, Zhenyu; Wang, Mengting; Ye, Meirong; Zhang, Benhou; Xue, Qingyun; Liu, Wei; Ding, Xiaoyu
• Is Part Of: Ecology and evolution. Volume 10:Issue 12(2020)
• Page Start: 5332
• Page End: 5342
• Publication Date: 2020-04-30
• Subjects: comparative plastomic approaches -- cpSSR -- Dendrobium huoshanense -- hotspot regions -- phylogeographic study; Ecology -- Periodicals; Evolution -- Periodicals; 577.05
• DOI: 10.1002/ece3.6277
• Languages: English
• ISSNs: 2045-7758

Abstract

Abstract: Comparative plastomics approaches have been used to identify available molecular markers for different taxonomic level studies of orchid species. However, the adoption of such methods has been largely limited in phylogeographic studies. Therefore, in this study, Dendrobium huoshanense, an endangered species with extremely small populations, was used as a model system to test whether the comparative plastomic approaches could screen available molecular markers for the phylogeographic study. We sequenced two more plastomes of D . huoshanense and compared them with our previously published one. A total of 27 mutational hotspot regions and six polymorphic cpSSRs have been screened for the phylogeographic studies of D . huoshanense . The cpDNA haplotype data revealed that the existence of haplotype distribution center was located in Dabieshan Mts. (Huoshan). The genetic diversity and phylogenetic analyses showed that the populations of D . huoshanense have been isolated and evolved independently for long period. On the contrary, based on cpSSR data, the genetic structure analysis revealed a mixed structure among the populations in Anhui and Jiangxi province, which suggested that the hybridization or introgression events have occurred among the populations of D. huoshanense . These results indicated that human activities have played key roles in shaping the genetic diversity and distributional patterns of D. huoshanense . According to our results, both two markers showed a high resolution for the phylogeographic studies of D . huoshanense . Therefore, we put forth that comparative plastomic approaches could revealed available molecular markers for phylogeographic study, especially for the species with extremely small populations. Abstract : Based on the comparative plastomic approaches, a total of 27 mutational hotspot regions and six polymorphic cpSSRs have been selected to assess the genetic diversity and distributional patterns of D. huoshanense . According to our results, both two markers showed a high resolution for the phylogeographic studies of D. huoshanense .