Influence of a cell salvage washing system and leukocyte reduction filtration on bacterial contamination of canine whole blood ex vivo. Issue 5 (12th March 2020)

• Record Type: Journal Article
• Authors: Hinson, Whitney D.; Rogovskyy, Artem S.; Lawhon, Sara D.; Thieman Mankin, Kelley M.
• Is Part Of: Veterinary surgery. Volume 49:Issue 5(2020)
• Page Start: 989
• Page End: 996
• Publication Date: 2020-03-12
• Subjects: Veterinary surgery -- Periodicals; Veterinary Medicine -- Periodicals; Surgery -- Periodicals; Societies, Medical -- Periodicals; 636.0897
• DOI: 10.1111/vsu.13410
• Languages: English
• ISSNs: 0161-3499

Abstract

Abstract: Objective: To determine the ability of cell salvage washing and leukoreduction filtration to remove bacterial contamination from canine whole blood. Study design: Ex vivo nested cohort study. Sample population: Commercially purchased fresh canine whole blood (n = 33 units). Methods: Commercially obtained canine whole blood was inoculated with known concentrations of one of three species of bacteria, Escherichia coli (ATCC 25922), Staphylococcus pseudintermedius (quality control strain; Texas A&M University), or Pseudomonas aeruginosa (ATCC 27853). Negative controls were inoculated with sterile saline. The inoculated blood was processed through a cell salvage system and filtered through a series of two leukocyte reduction filters. Samples were aseptically collected at five points during processing (inoculum, prewash, postwash, post–first filtration, and post–second filtration) for bacterial enumeration. Results: Bacterial concentrations were reduced by 85.2%, 91.5%, and 93.9% for E coli, S pseudintermedius, and P aeruginosa, respectively, after washing ( P < .0001), and bacterial concentrations were reduced by 99.9%, 100%, and 100%, respectively, after the first filtration ( P < .0001). After the second filtration, none of the three species of bacteria could be isolated (100% reduction). No bacterial growth was obtained from negative controls throughout the study. The type of bacteria ( P = .29) did not allow prediction of bacterial reduction. Conclusion: Cell salvage washing combined with leukoreduction filtration eliminated bacterial contamination of whole dog blood ( P < .0001). Clinical significance: Cell salvage washing and leukoreduction filtration could be applied to intraoperative autotransfusion in clinical animals, especially those treated for trauma or hemorrhage with concurrent bacterial contamination.