Generation of Footprint‐Free Canine Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus Vector. Issue 6 (19th May 2020)

• Record Type: Journal Article
• Authors: Tsukamoto, Masaya; Kimura, Kazuto; Tanaka, Miyuu; Kuwamura, Mitsuru; Ohtaka, Manami; Nakanishi, Mahito; Sugiura, Kikuya; Hatoya, Shingo
• Is Part Of: Molecular reproduction and development. Volume 87:Issue 6(2020)
• Page Start: 663
• Page End: 665
• Publication Date: 2020-05-19
• Subjects: canine induced pluripotent stem cells -- peripheral blood mononuclear cells -- Sendai virus vector -- SeVdp(KOSM)302L; Reproduction -- Periodicals; Molecular biology -- Periodicals; Molecular genetics -- Periodicals; Embryology -- Periodicals; 571.8
• DOI: 10.1002/mrd.23349
• Languages: English
• ISSNs: 1040-452X

Abstract

Abstract : Using auto‐erasable Sendai virus vector, we generated ciPSC line. After several passages, virus was not present in ciPSCs by RT‐PCR. ciPSCs from canine PBMCs had pluripotent state, differentiated all three germ layers in vitro, and had normal 78 XX karyotype. These results proved that PBMCs were one of the good cell sources to generate ciPSC lines from companion and patient dogs.