Studies on reproductive strategies of Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) based on morphological characteristics and SSR markers. Issue 12 (22nd April 2020)

• Record Type: Journal Article
• Authors: Sun, Xiaohan; Wang, Feng; Cui, Rong; Liu, Xiao; Li, Xiangxiang; Dong, Jibin; Sun, Lu; Qin, Siqi; Wang, Renqing; Zheng, Peiming; Wang, Hui
• Is Part Of: Ecology and evolution. Volume 10:Issue 12(2020)
• Page Start: 5270
• Page End: 5280
• Publication Date: 2020-04-22
• Subjects: mating system -- morphological characteristics -- reproductive strategies -- SSR markers -- Vitex negundo var. heterophylla; Ecology -- Periodicals; Evolution -- Periodicals; 577.05
• DOI: 10.1002/ece3.6271
• Languages: English
• ISSNs: 2045-7758

Abstract

Abstract: Vitex negundo L. var. heterophylla (Franch.) Rehder (Lamiaceae) is an important tree species for soil and water conservation, yet the reproductive ecology of this species remains to be elucidated. To investigate the reproductive traits of V. negundo var. heterophylla, the phenology, morphological characteristics (a suite of characters was assessed: floral morphology, nectar production, pollen viability, and stigma receptivity) and mating system of this species were systematically revealed for the first time in this study. Phenological observations, morphological measurements, and nectar production analysis were conducted during anthesis. Pollen viability and stigma receptivity at different flowering stages were measured by biochemical methods. Finally, genetic analysis based on SSR markers was used to reveal the mating system; outcrossing index and pollen‐ovule ratio were also calculated to help analysis. V. negundo var. heterophylla showed several obvious characteristics of outcrossing, such as abundant and attractive flowers, secreting nectar, and emitting scent. In addition, mechanisms such as homogamy and a short anther‐stigma distance that can promote self‐fertilization were also identified in this species. The coexistence of selfing and outcrossing characteristics demonstrates a predominantly outcrossed mixed mating system (outcrossing rate, t = 95%). The scientific information provided by this study may contribute to conservation of V. negundo var. heterophylla from a reproductive perspective. Abstract : To investigate the reproductive traits of Vitex negundo var. heterophylla and propose conservation strategies, the phenology, morphological characteristics, and mating system of this species were systematically revealed for the first time in this study. V. negundo var. heterophylla presents a predominantly outcrossed mixed mating system with self‐compatibility and inbreeding depression at the seedling emergence stage. Conservation strategies such as selective thinning and effective in‐suit protection should be carried out to ensure the stability of the population.