Immunological outcomes in infants with ROP after dexamethasone and aminophylline. (16th April 2020)

• Record Type: Journal Article
• Authors: Balashova, Larisa; Bykovskaya, Svetlana; Korobova, Ludmila; Kuznetsova, Yulia; Kantardgy, Elena; Mukhin, Vladimir; Popov, Andrey
• Is Part Of: Clinical and experimental pharmacology and physiology. Volume 47:Number 8(2020)
• Page Start: 1368
• Page End: 1373
• Publication Date: 2020-04-16
• Subjects: aminophylline -- dexamethasone -- immunosuppressant -- premature babies with retinopathy -- regulatory T cells -- sevoflurane general anaesthesia; Clinical pharmacology -- Periodicals; Pharmacology, Experimental -- Periodicals; Physiology, Experimental -- Periodicals; Physiology, Pathological -- Periodicals; 615.1
• DOI: 10.1111/1440-1681.13308
• Languages: English
• ISSNs: 0305-1870

Abstract

Abstract: This research aims to improve anaesthesia services given to preterm infants by the use of dexamethasone and aminophylline administrated under sevoflurane, and to analyze its effect on the cell‐mediated immunity (CD4+CD25+Foxp3+(T‐reg) and CD4+CD25highFoxp3+CD127low). We have examined 74 premature babies with retinopathy of prematurity (ROP) at the 3–5 stages during the 25–32 week gestation period (1–6 months after birth). Both immunomodulators had no significant effect on clinical parameters after one dose ( P > .05). Aminophylline (2.4% solution, 0.1 mL/kg or 0.132 mL per infant on average) and dexamethasone (0.4% solution, 0.1 mg/kg or 0.132 mL per infant on average) were intravenously injected 15 minutes before the end of the surgery. Required anaesthesia depth was maintained with inhalation anaesthetic (1.5–2.0 IAC), and the minimum fresh gas flow was not less than 2 L. Blood samples were taken from the vein (anaesthesia induction stage) into the tubes containing EDTA (the anticoagulant), stored at 20–25°C, and then, processed and stained within 24 hours after sampling. Both immunomodulators had no significant effect on clinical parameters after one dose ( P > .05). Short‐term shift in regulatory T‐cell level affected by dexamethasone has a negative effect combined with further withdrawal effect that this hormonal drug has. Aminophylline has such clinical effects as improving pulmonary ventilation, decrease in apnoea frequency, and improving blood gas indices. Aminophylline has less expressed but more prolonged positive effect during the day when used for several days. It may lead to a persistent positive effect with progressive treatment outcomes.