Design of novel, simple, and inexpensive 3D printing‐based miniaturized electrochemical platform containing embedded disposable detector for analytical applications. Issue 5 (30th September 2019)

• Record Type: Journal Article
• Authors: de Moraes, Natalia Canhete; da Silva, Eiva Natiele Tiago; Petroni, Jacqueline Marques; Ferreira, Valdir Souza; Lucca, Bruno Gabriel
• Editors: Foret, Frantisek; Kutter, Jörg
• Is Part Of: Electrophoresis. Volume 41:Issue 5/6(2020)
• Page Start: 278
• Page End: 286
• Publication Date: 2019-09-30
• Subjects: biological analysis -- carbon electrode -- clinical biomarkers -- electroanalysis -- voltammetry; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201900270
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: This paper describes the development of a novel, simple, and inexpensive electrochemical device containing an integrated and disposable three‐electrode system for detection. The base of this platform consists on a PDMS structure containing microchannels which were prototyped using 3D‐printed molds. Pencil graphite leads were inserted into these microchannels and utilized as working, counter and reference electrodes in a novel design. Morphological analysis and electrochemical experiments with benchmark redox probes were carried out in order to evaluate the performance and characterize the miniaturized device proposed. Even using inexpensive materials and a simple fabrication protocol, the electrochemical platform developed provided good repeatability and reproducibility over a low cost (ca. $2 per device), acceptable lifetime (ca. 250 voltammetric runs) and extremely reduced consumption of samples and reagents (order of µL). As proof of concept, the analytical feasibility of the platform was investigated through the simultaneous determination of dopamine (DOPA) and acetaminophen (AC). The two analytes showed linear dependence on the concentration range from 1 to 15 µM and the LODs achieved were 0.21 µM for DOPA and 0.29 µM for AC. Moreover, the platform was successfully applied on the determination of DOPA and AC in spiked blood serum and urine samples. The results obtained with the device described here were better than some reports in literature that use more costly electrodic materials and complex modification steps for the detection of the same analytes.