Characterization and in vitro assembly of tick‐borne encephalitis virus C protein. Issue 12 (22nd June 2020)

• Record Type: Journal Article
• Authors: Kaufman, Filip; Dostálková, Alžběta; Pekárek, Lukáš; Thanh, Tung Dinh; Kapisheva, Marina; Hadravová, Romana; Bednárová, Lucie; Novotný, Radim; Křížová, Ivana; Černý, Jiří; Grubhoffer, Libor; Ruml, Tomáš; Hrabal, Richard; Rumlová, Michaela
• Is Part Of: FEBS letters. Volume 594:Issue 12(2020)
• Page Start: 1989
• Page End: 2004
• Publication Date: 2020-06-22
• Subjects: assembly -- C protein -- DENV -- flavivirus -- protein‐nucleic acid interaction -- TBEV; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular biology -- Periodicals; Biochimie -- Périodiques; Biochemistry; Biophysics; Molecular biology; Periodicals; 572.05
• DOI: 10.1002/1873-3468.13857
• Languages: English
• ISSNs: 0014-5793

Abstract

Abstract : Tick‐borne encephalitis virus (TBEV), a member of flaviviruses, represents a serious health threat by causing human encephalitis mainly in central and eastern Europe, Russia, and northeastern Asia. As no specific therapy is available, there is an urgent need to understand all steps of the TBEV replication cycle at the molecular level. One of the critical events is the packaging of flaviviral genomic RNA by TBEV C protein to form a nucleocapsid. We purified recombinant TBEV C protein and used a combination of physical–chemical approaches, such as size‐exclusion chromatography, circular dichroism, NMR spectroscopies, and transmission electron microscopy, to analyze its structural stability and its ability to dimerize/oligomerize. We compared the ability of TBEV C protein to assemble in vitro into a nucleocapsid‐like structure with that of dengue C protein. Abstract :