Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. (7th May 2020)

• Record Type: Journal Article
• Authors: Trifunović‐Momčilov, Milana; Paunović, Danijela; Milošević, Snežana; Marković, Marija; Jevremović, Slađana; Dragićević, Ivana Č; Subotić, Angelina
• Is Part Of: Annals of applied biology. Volume 177:Number 1(2020)
• Page Start: 74
• Page End: 89
• Publication Date: 2020-05-07
• Subjects: AtCKX -- centaury -- cytokinins -- NaCl -- oxidative stress -- reactive oxygen species -- regeneration in vitro; Crop science -- Periodicals; Plants, Protection of -- Periodicals; Crops -- Ecology -- Periodicals; 630
• DOI: 10.1111/aab.12593
• Languages: English
• ISSNs: 0003-4746

Abstract

Abstract: Common centaury ( Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non‐transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non‐transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non‐transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2 ‐26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions. Abstract : Shoots and roots of non‐transformed and three AtCKX transgenic centaury lines were evaluated to salinity tolerance on graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Centaury roots showed higher tolerance to salinity than shoots. Morphogenic potential of AtCKX1 transgenic line was similar to non transformed line. Significant difference in morphogenic response of two investigated AtCKX2 transgenic lines were also determined. The addition of NaCl in culture medium elevated free proline, malondialdehyde, hydrogen peroxide content as well as antioxidative enzymes activities in all analysed centaury shoots and in roots.