Analysis of deformable distortion in the architecture of leaf xylary vessel elements of Carthamus oxycantha caused by heavy metals stress using image registration. Issue 7 (31st March 2020)

• Record Type: Journal Article
• Authors: Iqbal, Tahir; Shah, Said K.; Ullah, Faizan; Mehmood, Sultan; Zeb, Muhammad A.
• Is Part Of: Microscopy research and technique. Volume 83:Issue 7(2020)
• Page Start: 843
• Page End: 849
• Publication Date: 2020-03-31
• Subjects: anatomical study -- image registration -- warping -- xylary vessel; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23476
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Anatomical study of leaf xylary vessel elements of Carthamus oxycantha under various intensities of lead (Pb) and nickel (Ni) stress (200, 400, 600, and 800 mg Pb(NO3 )2, NiCl2 ·6H2 O/kg of the soil) was conducted. The deformations caused due to metal stress were detected using point‐based image registration technique. Initially, a set of corresponding feature points called landmarks was selected for warping of two‐dimensional microscopic images of deformed/source vessel (stressed) to its normal/target (unstressed) counterpart. The results show that the target registration error is less than 3 mm using real plant image datasets. The stress caused alterations mainly in diameter, size, and shape of the cells. Average cell diameter and average wall diameter of vessels were measured with "Image J." The range of decrease in average cell diameter from 18.566 to 13.1 μm and the range of increase in average wall diameter was from 5.166 to 10.1 μm, with increase in stress factor through 200, 400, 600, and 800 mg Pb(NO3 )2, NiCl2 ·6H2 O/kg of the soil. We noted large deformation in the form of shrinkage in cell size and diminution in its diameter. The diminution in diameter and the shrinkage in cell size of vessel cells may be due to the deposition of wall materials. It can be a possible strategy to limit the water flow to overcome the rapid mobility and transportation of the excess amount of metals to safeguard the cellular components from unpleasant consequences of metallic stress. Abstract : Anatomical study of leaf xylary vessel elements of Carthamus oxycantha under lead (Pb) and nickel (Ni) stress was conducted. The deformations caused due to metals stress were detected using point‐based nonrigid image registration technique. The 3DWarpDx (an algorithm based software technique) was applied to compare microscopic images of stressed and unstressed vessels. The technique determined the soft deformable anatomical distortion in structure of the stressed vessels. We noted large deformation in the form of shrinkage in cell size and diminution in its diameter.