The influence of GAPT extraction on synapse loss of APPswe/PS1dE9 transgenic mice via adjusting Bcl‐2/Bax balance. Issue 1 (21st November 2018)

• Record Type: Journal Article
• Authors: Shi, Jing; Zhang, Xuekai; Ni, Jingnian; Wei, Mingqing; Li, Ting; Zhou, Bingling; Liu, Xiawei; Zhang, Liping; Wang, Pengwen; Tian, Jinzhou; Wang, Yongyan
• Is Part Of: Alzheimer's & dementia. Volume 4:Issue 1(2018)
• Page Start: 724
• Page End: 736
• Publication Date: 2018-11-21
• Subjects: Alzheimer's disease -- APP/PS1 transgenic mice -- Aβ deposition -- GAPT -- Bcl‐2/Bax balance -- Neuron apoptosis; Dementia -- Periodicals; Dementia -- Treatment -- Periodicals; Alzheimer's disease -- Treatment -- Periodicals; Alzheimer's disease -- Periodicals; 616.831
• DOI: 10.1016/j.trci.2018.10.008
• Languages: English
• ISSNs: 2352-8737

Abstract

Abstract: Introduction: The degeneration of memory‐focused synapses play important roles in Alzheimer's disease (AD) pathogenesis, while it is not well known how β amyloid interferes neuron apoptosis and how a herbal combination GAPT influence synapse loss and neuronal apoptosis pathways of APP/PS1 transgenic mice. Methods: Three‐month and six‐month APPswe/PS1dE9 transgenic mice were used. Spatial and memory ability were measured by Morris Water Maze, Neuron and synapse number were assessed by electron microscope; Aβ, Bcl‐2/Bax were determined by immunohistochemistry and western blot. Results: APP/PS1 mice not only had increased Aβ accumulation, impaired memory performance, less synapse number, and much more necrosed neurons, but also had significant reduction in the Bcl‐2/Bax ratio. However, GAPT and donepezil showed improved memory performance, less Aβ accumulation, increased neuron and synapse number, as well as restored balance of Bcl‐2/Bax. Discussion: GAPT may improve cognitive functions via both reducing Aβ deposition and restoring Bcl‐2/Bax balance of neuron.