Arrested cell proliferation through cysteine protease activity of eukaryotic ribosomal protein S4. Issue 2 (1st November 2012) - Yadaiah, Madasu
Abstract : S4 is an integral protein of the smaller subunit of cytosolic ribosome. In prokaryotes, it regulates the synthesis of ribosomal proteins by feedback inhibition of the α‐operon gene expression, and it facilitates ribosomal RNA synthesis by direct binding to RNA polymerase. However, functional roles of S4 in eukaryotes are poorly understood, although its deficiency in humans is thought to produce Turner syndrome. We report here that wheat S4 is a cysteine protease capable of abrogating total protein synthesis in an actively translating cell‐free system of rabbit reticulocytes. The translation‐blocked medium, imaged by atomic force microscopy, scanning electron microscopy, and transmission electron microscopy, shows dispersed polysomes, and the disbanded polyribosome elements aggregate to form larger bodies. We also show that human embryonic kidney cells transfected with recombinant wheat S4 are unable to grow and proliferate. The mutant S4 protein, where the putative active site residue Cys 41 is replaced by a phenylalanine, can neither suppress protein synthesis nor arrest cell proliferation, suggesting that the observed phenomenon arises from the cysteine protease attribute of S4. The results also inspire many questions concerning in vivo significance of extraribosomal roles of eukaryotic S4 performed through its protease activity.—Yadaiah, M., Sudhamalla, B., Rao, P. N., Roy, K. R., Ramakrishna, D., Hussain Syed, G., Ramaiah, K. V. A., Bhuyan, A. K. Arrested cell proliferation through cysteine protease activity of eukaryotic ribosomal protein S4. FASEB J. 27, 803–810 (2013). www.fasebj.org