IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells via Wnt/β‐catenin signaling pathway. Issue 4 (5th January 2018)
- Record Type:
- Journal Article
- Title:
- IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells via Wnt/β‐catenin signaling pathway. Issue 4 (5th January 2018)
- Main Title:
- IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells via Wnt/β‐catenin signaling pathway
- Authors:
- Zhang, Wei
Chen, Erman
Chen, Mo
Ye, Chenyi
Qi, Yiying
Ding, Qianhai
Li, Hang
Xue, Deting
Gao, Xiang
Pan, Zhijun - Abstract:
- Abstract : Insulin‐like growth factor‐binding protein 7 (IGFBP7), a low‐affinity IGF binder, may play an important role in bone metabolism. However, its function in osteogenic differentiation of human bone marrow‐derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we investigated its effects on osteogenic differentiation. Overexpression of IGFBP7 enhanced the expression of osteo‐specific genes and proteins, and IGFBP7 knockdown decreased osteogenesis‐specific markers. More mineral deposits and higher alkaline phosphatase activity were observed after the up‐regulation of IGFBP7 . Moreover, β‐catenin levels were up‐regulated by the overexpression of IGFBP7 or the addition of extracellular IGFBP7 protein and were reduced by the depletion of IGFBP7 . The increase in osteogenic differentiation due to the overexpression of IGFBP7 was partially decreased by specific Wnt/β‐catenin signaling inhibitors. Using a rat tibial osteotomy model, a sheet of IGFBP7–overexpressing BMSCs improved bone healing, as demonstrated by imaging, biomechanical, and histologic analyses. Taken together, these findings indicate that IGFBP7 regulates the osteogenic differentiation of BMSCs partly via the Wnt/ β‐catenin signaling pathway.— Zhang, W., Chen, E., Chen, M., Ye, C., Qi, Y., Ding, Q., Li, H., Xue, D., Gao, X., Pan, Z. IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells via Wnt/β‐catenin signaling pathway. FASEB J. 32, 2280–2291 (2018).Abstract : Insulin‐like growth factor‐binding protein 7 (IGFBP7), a low‐affinity IGF binder, may play an important role in bone metabolism. However, its function in osteogenic differentiation of human bone marrow‐derived mesenchymal stem cells (BMSCs) remains unclear. Therefore, we investigated its effects on osteogenic differentiation. Overexpression of IGFBP7 enhanced the expression of osteo‐specific genes and proteins, and IGFBP7 knockdown decreased osteogenesis‐specific markers. More mineral deposits and higher alkaline phosphatase activity were observed after the up‐regulation of IGFBP7 . Moreover, β‐catenin levels were up‐regulated by the overexpression of IGFBP7 or the addition of extracellular IGFBP7 protein and were reduced by the depletion of IGFBP7 . The increase in osteogenic differentiation due to the overexpression of IGFBP7 was partially decreased by specific Wnt/β‐catenin signaling inhibitors. Using a rat tibial osteotomy model, a sheet of IGFBP7–overexpressing BMSCs improved bone healing, as demonstrated by imaging, biomechanical, and histologic analyses. Taken together, these findings indicate that IGFBP7 regulates the osteogenic differentiation of BMSCs partly via the Wnt/ β‐catenin signaling pathway.— Zhang, W., Chen, E., Chen, M., Ye, C., Qi, Y., Ding, Q., Li, H., Xue, D., Gao, X., Pan, Z. IGFBP7 regulates the osteogenic differentiation of bone marrow‐derived mesenchymal stem cells via Wnt/β‐catenin signaling pathway. FASEB J. 32, 2280–2291 (2018). www.fasebj.org … (more)
- Is Part Of:
- FASEB journal. Volume 32:Issue 4(2018)
- Journal:
- FASEB journal
- Issue:
- Volume 32:Issue 4(2018)
- Issue Display:
- Volume 32, Issue 4 (2018)
- Year:
- 2018
- Volume:
- 32
- Issue:
- 4
- Issue Sort Value:
- 2018-0032-0004-0000
- Page Start:
- 2280
- Page End:
- 2291
- Publication Date:
- 2018-01-05
- Subjects:
- IGFBP7 -- BMSCs -- osteogenesis
Biology -- Periodicals
Biology, Experimental -- Periodicals
570 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1096/fj.201700998RR ↗
- Languages:
- English
- ISSNs:
- 0892-6638
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13310.xml