Prostate cancer cell migration induced by myopodin isoforms is associated with formation of morphologically and biochemically distinct actin networks. Issue 12 (4th September 2013)
- Record Type:
- Journal Article
- Title:
- Prostate cancer cell migration induced by myopodin isoforms is associated with formation of morphologically and biochemically distinct actin networks. Issue 12 (4th September 2013)
- Main Title:
- Prostate cancer cell migration induced by myopodin isoforms is associated with formation of morphologically and biochemically distinct actin networks
- Authors:
- Kai, FuiBoon
Duncan, Roy - Abstract:
- Abstract : Myopodin is an actin‐binding protein that promotes cancer cell migration in response to serum stimulation and is associated with invasive tumor development. To determine whether enhanced migration reflects changes in actin cytoskeleton remodeling, fluorescence confocal microscopy was used to examine the composition and morphology of filamentous actin structures in mock‐transduced cells vs. stably transduced PC3 cells expressing human myopodin isoforms, and the chemokinetic response of cells was quantified using transwell assays. The same approaches were used to analyze the effects of external migration stimuli, actin polymerization inhibitors or deletion of the isoform‐specific amino‐ and/or carboxy termini on cell migration and actin bundle formation. Results indicate that the termini of the myopodin isoforms differentially alter the formation of morphologically distinct F‐actin networks that also differ in their myosin and myopodin staining patterns. Furthermore, enhanced cell migration was reduced by > 50% when actin bundle formation was impaired by myopodin‐truncation, low concentrations of an actin polymerization inhibitor, or in the absence of an external migration stimulus. Human myopodin isoforms are therefore potent regulators of stress fiber formation, inducing the formation of biochemically and morphologically distinct F‐actin networks in the cell body whose presence directly correlates with increased cell migration.—Kai, F., Duncan, R., Prostate cancerAbstract : Myopodin is an actin‐binding protein that promotes cancer cell migration in response to serum stimulation and is associated with invasive tumor development. To determine whether enhanced migration reflects changes in actin cytoskeleton remodeling, fluorescence confocal microscopy was used to examine the composition and morphology of filamentous actin structures in mock‐transduced cells vs. stably transduced PC3 cells expressing human myopodin isoforms, and the chemokinetic response of cells was quantified using transwell assays. The same approaches were used to analyze the effects of external migration stimuli, actin polymerization inhibitors or deletion of the isoform‐specific amino‐ and/or carboxy termini on cell migration and actin bundle formation. Results indicate that the termini of the myopodin isoforms differentially alter the formation of morphologically distinct F‐actin networks that also differ in their myosin and myopodin staining patterns. Furthermore, enhanced cell migration was reduced by > 50% when actin bundle formation was impaired by myopodin‐truncation, low concentrations of an actin polymerization inhibitor, or in the absence of an external migration stimulus. Human myopodin isoforms are therefore potent regulators of stress fiber formation, inducing the formation of biochemically and morphologically distinct F‐actin networks in the cell body whose presence directly correlates with increased cell migration.—Kai, F., Duncan, R., Prostate cancer cell migration induced by myopodin isoforms is associated with formation of morphologically and biochemically distinct actin networks. FASEB J. 27, 5046–5058 (2013). www.fasebj.org … (more)
- Is Part Of:
- FASEB journal. Volume 27:Issue 12(2013)
- Journal:
- FASEB journal
- Issue:
- Volume 27:Issue 12(2013)
- Issue Display:
- Volume 27, Issue 12 (2013)
- Year:
- 2013
- Volume:
- 27
- Issue:
- 12
- Issue Sort Value:
- 2013-0027-0012-0000
- Page Start:
- 5046
- Page End:
- 5058
- Publication Date:
- 2013-09-04
- Subjects:
- PC3 cells -- actin bundles -- stress fibers -- actomyosin -- synaptopodin 2
Biology -- Periodicals
Biology, Experimental -- Periodicals
570 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1096/fj.13-231571 ↗
- Languages:
- English
- ISSNs:
- 0892-6638
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13320.xml