Cyclophilin A contributes to aortopathy induced by postnatal loss of smooth muscle TGFBR1. Issue 10 (16th July 2019)

• Record Type: Journal Article
• Authors: Zhou, Guannan; Liao, Mingmei; Wang, Fen; Qi, Xiaoyan; Yang, Pu; Berceli, Scott A.; Sharma, Ashish K.; Upchurch, Gilbert R.; Jiang, Zhihua
• Is Part Of: FASEB journal. Volume 33:Issue 10(2019)
• Page Start: 11396
• Page End: 11410
• Publication Date: 2019-07-16
• Subjects: AAD -- receptor -- growth factor; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.201900601RR
• Languages: English
• ISSNs: 0892-6638

Abstract

ABSTRACT: Recent recognition that TGF‐β signaling disruption is involved in the development of aortic aneurysms has led to renewed investigations into the role of TGF‐β biology in the aortic wall. We previously found that the type I receptor of TGF‐β (TGFBR2) receptor contributes to formation of ascending aortic aneurysms and dissections (AADs) induced by smooth muscle cell (SMC)‐specific, postnatal deletion of Tgfbr1 (Tgfbr1 iko ). Here, we aimed to decipher the mechanistic signaling pathway underlying the pathogenic effects of TGFBR2 in this context. Gene expression profiling demonstrated that Tgfbr1 iko triggers an acute inflammatory response in developing AADs, and Tgfbr1 iko SMCs express an inflammatory phenotype in culture. Comparative proteomics profiling and mass spectrometry revealed that Tgfbr1 iko SMCs respond to TGF‐β1 stimulation via robust up‐regulation of cyclophilin A (CypA). This up‐regulation is abrogated by inhibition of TGFBR2 kinase activity, small interfering RNA silencing of Tgfbr2 expression, or inhibition of SMAD3 activation. In mice, Tgfbr1 iko rapidly promotes CypA production in SMCs of developing AADs, whereas treatment with a CypA inhibitor attenuates aortic dilation by 56% ( P = 0.003) and ameliorates aneurysmal degeneration ( P = 0.016). These protective effects are associated with reduced aneurysm‐promoting inflammation. Collectively, these results suggest a novel mechanism, wherein loss of type I receptor of TGF‐β triggers promiscuous, proinflammatory TGFBR2 signaling in SMCs, thereby promoting AAD formation.—Zhou, G., Liao, M., Wang, F., Qi, X., Yang, P., Berceli, S. A., Sharma, A. K., Upchurch, G. R., Jr., Jiang, Z. Cyclophilin A contributes to aortopathy induced by postnatal loss of smooth muscle TGFBR1. FASEB J. 33, 11396–11410 (2019). www.fasebj.org