Vitamin D improves endothelial barrier integrity and counteracts inflammatory effects on endothelial progenitor cells. Issue 8 (14th May 2019)

• Record Type: Journal Article
• Authors: Schröder‐Heurich, Bianca; von Hardenberg, Sandra; Brodowski, Lars; Kipke, Berina; von Meyer, Nadia; Borns, Katja; Kaisenberg, Constantin S.; Brinkmann, Hella; Claus, Peter; von Versen‐Höynck, Frauke
• Is Part Of: FASEB journal. Volume 33:Issue 8(2019)
• Page Start: 9142
• Page End: 9153
• Publication Date: 2019-05-14
• Subjects: endothelial colony‐forming cells -- TNF‐α adhesion proteins -- VE‐cadherin -- endothelial dysfunction; Biology -- Periodicals; Biology, Experimental -- Periodicals; 570
• DOI: 10.1096/fj.201802750RR
• Languages: English
• ISSNs: 0892-6638

Abstract

ABSTRACT: Endothelial colony‐forming cells (ECFCs), a proliferative subpopulation of endothelial progenitor cells, are involved in angiogenesis and endothelial repair. In this study, we investigated endothelial barrier characteristics of ECFCs, whether vitamin D supports cell‐cell adhesion and barrier integrity, and how it affects ECFC mobilization and actin dynamics. Although ECFC barrier was disrupted under inflammatory conditions, this effect was rescued by vitamin D treatment, leading to higher stability of an ECFC monolayer. Furthermore, vitamin D enhanced ECFC mobilization toward directional migration. In addition, immunocytochemistry, quantitative real‐time PCR, and immunoblotting analysis showed that vitamin D increased endothelial interconnections through vascular endothelial Cadherin (VE‐cadherin) junctions and by impacting cell dynamics through cofilin and VE‐cadherin phosphorylation. Our results suggest that vitamin D treatment efficiently counteracts inflammation in an ECFC monolayer, resulting in higher ECFC barrier integrity. This study provides evidence of a new beneficial effect of vitamin D for ECFC homeostasis. —Schröder‐Heurich, B., von Hardenberg, S., Brodowski, L., Kipke, B., Meyer, N., Borns, K., von Kaisenberg, C. S., Brinkmann, H., Claus, P., von Versen‐Höynck, F. Vitamin D improves endothelial barrier integrity and counteracts inflammatory effects on endothelial progenitor cells. FASEB J. 33, 9142–9153 (2019). www.fasebj.org