Populus euphratica WRKY1 binds the promoter of H+-ATPase gene to enhance gene expression and salt tolerance. (4th November 2019)

• Record Type: Journal Article
• Authors: Yao, Jun; Shen, Zedan; Zhang, Yanli; Wu, Xia; Wang, Jianhui; Sa, Gang; Zhang, Yuhong; Zhang, Huilong; Deng, Chen; Liu, Jian; Hou, Siyuan; Zhang, Ying; Zhang, Yinan; Zhao, Nan; Deng, Shurong; Lin, Shanzhi; Zhao, Rui; Chen, Shaoliang
• Editors: Murphy, Angus
• Is Part Of: Journal of experimental botany. Volume 71:Number 4(2020)
• Page Start: 1527
• Page End: 1539
• Publication Date: 2019-11-04
• Subjects: DNA affinity purification sequencing -- electrophoretic mobility shift assay -- H+-ATPase -- luciferase reporter assay -- NaCl -- non-invasive microtest technique -- PeWRKY1 -- virus-induced gene silencing -- W-box; Botany -- Periodicals; Botany, Experimental -- Periodicals; Plant physiology -- Periodicals; 580
• DOI: 10.1093/jxb/erz493
• Languages: English
• ISSNs: 0022-0957

Abstract

Abstract : Populus euphratica WRKY1 increases the expression of the plasma membrane H + -ATPase gene by binding the promoter of PeHA1 in the presence of salt stress. Abstract: Plasma membrane proton pumps play a crucial role in maintaining ionic homeostasis in salt-resistant Populus euphratica under saline conditions. High levels of NaCl (200 mM) induced PeHA1 expression in P. euphratica roots and leaves. We isolated a 2022 bp promoter fragment upstream of the translational start of PeHA1 from P. euphratica . The promoter–reporter construct PeHA1-pro :: GUS was transferred to tobacco plants, demonstrating that β-glucuronidase activities increased in root, leaf, and stem tissues under salt stress. DNA affinity purification sequencing revealed that PeWRKY1 protein targeted the PeHA1 gene. We assessed the salt-induced transcriptional response of PeWRKY1 and its interaction with PeHA1 in P. euphratica . PeWRKY1 binding to the PeHA1 W-box in the promoter region was verified by a yeast one-hybrid assay, EMSA, luciferase reporter assay, and virus-induced gene silencing. Transgenic tobacco plants overexpressing PeWRKY1 had improved expression of NtHA4, which has a cis -acting W-box in the regulatory region, and improved H + pumping activity in both in vivo and in vitro assays. We conclude that salt stress up-regulated PeHA1 transcription due to the binding of PeWRKY1 to the W-box in the promoter region of PeHA1 . Thus, we conclude that enhanced H + pumping activity enabled salt-stressed plants to retain Na + homeostasis.