Detecting metabolic differences in fetal and adult sheep adipose and skeletal muscle tissues. Issue 3 (23rd December 2019)

• Record Type: Journal Article
• Authors: Darby, Jack R. T.; Sorvina, Alexandra; Bader, Christie A.; Lock, Mitchell C.; Soo, Jia Yin; Holman, Stacey L.; Seed, Mike; Kuchel, Tim; Brooks, Douglas A.; Plush, Sally E.; Morrison, Janna L.
• Is Part Of: Journal of biophotonics. Volume 13:Issue 3(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-12-23
• Subjects: adipose -- fetus -- metabolism -- OXPHOS -- redox -- skeletal muscle -- two photon microscopy; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201960085
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract: The primary metabolic pathway required to produce ATP differs as a result of tissue type, developmental stage and substrate availability. We utilized molecular and histological techniques to define the metabolic status in foetal and adult, adipose and skeletal muscle tissues. Redox ratios of these tissues were also determined optically by two‐photon microscopy. Adult perirenal adipose tissue had a higher optical redox ratio than fetal perirenal adipose tissue, which aligned with glycolysis being used for ATP production; whereas adult skeletal muscle had a lower optical redox ratio than fetal skeletal muscle, which aligned with oxygen demanding oxidative phosphorylation activity being utilized for ATP production. We have compared traditional molecular and microscopy techniques of metabolic tissue characterization with optical redox ratios to provide a more comprehensive report on the dynamics of tissue metabolism. Abstract : Optical redox ratios obtained through two‐photon microscopy align with metabolic mRNA expression profiles as well as lipid and mitochondrial distribution to differentiate between brown and white adipose tissue.