Stress‐ and pathway‐specific impacts of impaired jasmonoyl‐isoleucine (JA‐Ile) catabolism on defense signalling and biotic stress resistance. (25th March 2020)

• Record Type: Journal Article
• Authors: Marquis, Valentin; Smirnova, Ekaterina; Poirier, Laure; Zumsteg, Julie; Schweizer, Fabian; Reymond, Philippe; Heitz, Thierry
• Is Part Of: Plant, cell and environment. Volume 43:Number 6(2020)
• Page Start: 1558
• Page End: 1570
• Publication Date: 2020-03-25
• Subjects: Arabidopsis -- botrytis -- catabolism -- defense -- feedback -- jasmonate -- resistance -- signalling -- wounding; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.13753
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Jasmonate synthesis and signalling are essential for plant defense upregulation upon herbivore or microbial attacks. Stress‐induced accumulation of jasmonoyl‐isoleucine (JA‐Ile), the bioactive hormonal form triggering transcriptional changes, is dynamic and transient because of the existence of potent removal mechanisms. Two JA‐Ile turnover pathways operate in Arabidopsis, consisting in cytochrome P450 (CYP94)‐mediated oxidation and deconjugation by the amidohydrolases IAR3/ILL6. Understanding their impacts was previously blurred by gene redundancy and compensation mechanisms. Here we address the consequences of blocking these pathways on jasmonate homeostasis and defenses in double‐ 2ah, triple‐ 3cyp mutants, and a quintuple ‐5ko line deficient in all known JA‐Ile‐degrading activities. These lines reacted differently to either mechanical wounding/insect attack or fungal infection. Both pathways contributed additively to JA‐Ile removal upon wounding, but their impairement had opposite impacts on insect larvae feeding. By contrast, only the ah pathway was essential for JA‐Ile turnover upon infection by Botrytis, yet only 3cyp was more fungus‐resistant. Despite building‐up extreme JA‐Ile levels, 5ko displayed near‐wild‐type resistance in both bioassays. Molecular analysis indicated that restrained JA‐Ile catabolism resulted in enhanced defense/resistance only when genes encoding negative regulators were not simultaneously overstimulated. This occurred in discrete stress‐ and pathway‐specific combinations, providing a framework for future defense‐enhancing strategies. Abstract : We investigated the impacts of impairing catabolic pathways of the defense hormone jasmonoyl‐isoleucine in higher order mutants of Arabidopsis thaliana . The data disclosed pathway‐specific changes on resistance to herbivory or fungal infection that correlated with feedback inhibition responses.