A Cofactor‐Substrate‐Based Supramolecular Fluorescent Probe for the Ultrafast Detection of Nitroreductase under Hypoxic Conditions. (21st February 2020)

• Record Type: Journal Article
• Authors: Jiao, Yang; Zhang, Lei; Gao, Xu; Si, Wen; Duan, Chunying
• Is Part Of: Angewandte Chemie. Volume 132:Number 15(2020)
• Page Start: 6077
• Page End: 6083
• Publication Date: 2020-02-21
• Subjects: Cofaktormimetika -- Enzyme -- Hypoxie -- Metallorganische Kapseln -- Supramolekulare Chemie; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201915040
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Identifying the location and expression levels of enzymes under hypoxic conditions in cancer cells is vital in early‐stage cancer diagnosis and monitoring. By encapsulating a fluorescent substrate, L‐NO2, within the NADH mimic‐containing metal–organic capsule Zn‐MPB, we developed a cofactor‐substrate‐based supramolecular luminescent probe for ultrafast detection of hypoxia‐related enzymes in solution in vitro and in vivo. The host–guest structure fuses the coenzyme and substrate into one supramolecular probe to avoid control by NADH, switching the catalytic process of nitroreductase from a double‐substrate mechanism to a single‐substrate one. This probe promotes enzyme efficiency by altering the substrate catalytic process and enhances the electron transfer efficiency through an intra‐molecular pathway with increased activity. The enzyme content and fluorescence intensity showed a linear relationship and equilibrium was obtained in seconds, showing potential for early tumor diagnosis, biomimetic catalysis, and prodrug activation. Abstract : Eine supramolekulare Sonde für den ultraschnellen (Sekunden) und quantitativen Nachweis von Nitroreduktase unter hypoxischen Bedingungen wurde entwickelt. Die Sonde verbessert die Enzymeffizienz durch Eingriff in den Substratkatalyseprozess und die Elektronentransfereffizienz durch einen intramolekularen Reaktionspfad mit erhöhter Aktivität.