Carotenoids in the eyespot apparatus are required for triggering phototaxis in Euglena gracilis. (3rd December 2019)

• Record Type: Journal Article
• Authors: Kato, Shota; Ozasa, Kazunari; Maeda, Mizuo; Tanno, Yuri; Tamaki, Shun; Higuchi‐Takeuchi, Mieko; Numata, Keiji; Kodama, Yutaka; Sato, Mayuko; Toyooka, Kiminori; Shinomura, Tomoko
• Is Part Of: Plant journal. Volume 101:Number 5(2020)
• Page Start: 1091
• Page End: 1102
• Publication Date: 2019-12-03
• Subjects: phototaxis -- eyespot -- carotenoid -- microfluidic device -- video tracking -- electron microscopy; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.14576
• Languages: English
• ISSNs: 0960-7412

Abstract

Summary: Carotenoids are the most universal and most widespread pigments in nature. They have played pivotal roles in the evolution of photosensing mechanisms in microbes and of vision in animals. Several groups of phytoflagellates developed a photoreceptive organelle called the eyespot apparatus (EA) consisting of two separable components: the eyespot, a cluster of carotenoid‐rich globules that acts as a reflector device, and actual photoreceptors for photobehaviors. Unlike other algal eyespots, the eyespot of Euglenophyta lacks reflective properties and is generally considered to act as a shading device for the photoreceptor (paraflagellar body, PFB) for major photomovements. However, the function of the eyespot of Euglenophyta has not yet been fully proven. Here, we report that the blocking carotenoid biosynthesis in Euglena gracilis by suppressing the phytoene synthase gene ( crtB ) caused a defect in eyespot function resulting in a loss of phototaxis. Raman spectroscopy and transmission electron microscopy suggested that Eg crtB ‐suppressed cells formed eyespot globules but had a defect in the accumulation of carotenoids in those packets. Motion analysis revealed the loss of phototaxis in Eg crtB ‐suppressed cells: a defect in the initiation of turning movements immediately after a change in light direction, rather than a defect in the termination of cell turning at the appropriate position due to a loss of the shading effect on the PFB. This study revealed that carotenoids are essential for light perception by the EA for the initiation of phototactic movement by E. gracilis, suggesting one possible photosensory role of carotenoids in the EA for the phototaxis. Significance Statement: The eyespot apparatus (EA) of Euglena gracilis consists of a cluster of cytosolic carotenoid‐rich globules, called the eyespot, and the actual photoreceptive organelle, the paraflagellar body (PFB). The eyespot of this alga has been speculated to be just a shading device for the PFB. This study revealed by single‐cell tracking techniques that the eyespot is necessary for phototaxis of this alga and that carotenoids participate in light perception by the EA to initiate phototactic movement.