Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers. (6th March 2020)

• Record Type: Journal Article
• Authors: Steinmetzger, Christian; Bäuerlein, Carmen; Höbartner, Claudia
• Is Part Of: Angewandte Chemie. Volume 132:Number 17(2020)
• Page Start: 6826
• Page End: 6830
• Publication Date: 2020-03-06
• Subjects: Fluoreszenzresonanz-Energietransfer -- Isomorphe Nukleobasen-Analoga -- RNA-Aptamere -- Stokes-Verschiebung -- Struktursonden; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201916707
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence‐based strategies reveal information on structure and dynamics of RNA aptamers. Herein, we report the incorporation of the universal emissive nucleobase analog 4‐cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to the bound ligands DMHBI + or DMHBO + . The photophysical properties of the new nucleobase–ligand‐FRET pair revealed structural restraints for the overall RNA aptamer organization and identified nucleotide positions suitable for FRET‐based readout of ligand binding. This strategy is generally suitable for binding‐site mapping and may also be applied for responsive aptamer devices. Abstract : Gespickte RNA : Das fluoreszierende Nukleobasen‐Analogon 4‐Cyanoindol wurde positionsspezifisch in das fluorogene Chili‐RNA‐Aptamer zur Beobachtung der Bindung verschiedener Liganden eingebaut. Diese erste Anwendung von FRET zwischen einem isomorphen Nukleobasendonor und einem intrinsisch fluorogenen Liganden lieferte Strukturinformationen über die Ligandenbindungsstelle anhand positionsabhängiger Quantenausbeuten und FRET‐Effizienzen.