Molecular shape under far‐red light and red light‐induced association of Arabidopsis phytochrome B. (31st October 2019)

• Record Type: Journal Article
• Authors: Oide, Mao; Hikima, Takaaki; Oroguchi, Tomotaka; Kato, Takayuki; Yamaguchi, Yuhki; Yoshihara, Shizue; Yamamoto, Masaki; Nakasako, Masayoshi; Okajima, Koji
• Is Part Of: FEBS journal. Volume 287:Number 8(2020)
• Page Start: 1612
• Page End: 1625
• Publication Date: 2019-10-31
• Subjects: light‐induced changes -- molecular shape -- phytochrome B -- size‐exclusion chromatography -- small‐angle X‐ray scattering; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.15095
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : Phytochrome B (phyB) is a plant photoreceptor protein that regulates various photomorphogenic responses to optimize plant growth and development. PhyB exists in two photoconvertible forms: a red light‐absorbing (Pr) and a far‐red light‐absorbing (Pfr) form. Therefore, to understand the mechanism of phototransformation, the structural characterization of full‐length phyB in these two forms is necessary. Here, we report the molecular structure of Arabidopsis thaliana phyB in Pr form and the molecular properties of the Pfr form determined by small‐angle X‐ray scattering coupled with size‐exclusion chromatography. In solution, the Pr form associated as a dimer with a radius of gyration of 50 Å. The molecular shape was a crossed shape, in which the orientation of the photosensory modules differed from that in the crystal structure of dimeric photosensory module. PhyB exhibited structural reversibility in the Pfr‐to‐Pr phototransformation and thermal reversion from Pfr to Pr in the dark. In addition, Pfr only exhibited nonspecific association, which distinguished molecular properties of Pfr form from those of the inactive Pr form. Abstract : Phytochrome B (phyB) is a plant photoreceptor protein that regulates various red/far‐red photomorphogenic responses. Despite the importance of phyB in plant growth and development, the structural details of full‐length phyB are still unknown. Here, based on analyses by small‐angle X‐ray scattering, we report the molecular structure regarding the arrangement of photosensory and output modules, and properties of Arabidopsis thaliana phyB.