Sequencing and characterization of mitochondrial DNA genome for Brama japonica (Perciformes: Bramidae) with phylogenetic consideration. (October 2016)

• Record Type: Journal Article
• Authors: Chen, Fenfang; Ma, Hongyu; Ma, Chunyan; Zhang, Heng; Zhao, Mengdi; Meng, Yongyong; Wei, Hongqing; Ma, Lingbo
• Is Part Of: Biochemical systematics and ecology. Volume 68(2016)
• Page Start: 109
• Page End: 118
• Publication Date: 2016-10
• Subjects: Brama japonica -- Mitochondrial genome -- Gene arrangement -- Light-strand replication origin -- Phylogenetic relationship; Chemotaxonomy -- Periodicals; Biochemical variation -- Periodicals; Ecology -- Periodicals; Biochemistry -- Periodicals; Ecology -- Periodicals; Chimiotaxinomie -- Périodiques; Variation biochimique -- Périodiques; Écologie -- Périodiques; 578.012
• DOI: 10.1016/j.bse.2016.06.012
• Languages: English
• ISSNs: 0305-1978

Abstract

Abstract: In this study, the complete mitochondrial genome sequence of Brama japonica is isolated and characterized by PCR and primer-walking sequencing techniques. The complete DNA is 17, 009 bp in length and contained 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes and a long putative control region. The gene organization and nucleotide composition are identical to those of other Bramidae fishes. In contrast, the 12S rRNA gene contains a big poly C structure which is larger than those from other Bramidae species. Of 37 genes, twenty-eight are encoded by heavy strand, while nine are encoded by light strand. Among the 13 protein-coding genes, twelve employ ATG as start codon, while only one (COI) utilizes GTG as start codon. The terminal associated sequence (TAS), the central and conserved sequence block (CSB-E and CSB-D) and a variable domain (CSB-1, CSB-2 and CSB-3) are identified in the control region, while the typical central conserved CSB-F is not detected. From the phylogenetic tree, we find that B. japonica, together with other five Bramidae species form a monophyletic group among 24 species. This work provides a set of useful data for studies on population genetic diversity and molecular evolution in Bramidae fish species. Highlights: The complete mitochondrial genome of Brama japonica is developed. This genome contains a typical 37 genes and a control region. Brama japonica is genetically closest to four Bramidae species.