A Novel Fluorescent Probe Based on Perylene Derivative for Hg2+ Ions and Biological Thiols and its Application in Live Cell Imaging and Theoretical Calculations. Issue 4 (19th December 2019)

• Record Type: Journal Article
• Authors: Karuk Elmas, Şükriye Nihan; Berk Gunay, Ibrahim; Karagoz, Abdurrahman; Bostanci, Aykut; Sadi, Gökhan; Yilmaz, Ibrahim
• Is Part Of: Electroanalysis. Volume 32:Issue 4(2020)
• Page Start: 775
• Page End: 780
• Publication Date: 2019-12-19
• Subjects: Perylene -- Fluorescence Sensor -- Mercury -- Cysteine -- Homocysteine -- Glutathione; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201900655
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: The selective and sensitive detection of biothiols; cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions is of considerable importance because of their pivotal roles in maintaining the reducing environment in the cells. This study describes a strategy for the determination of biothiols based on the PDI/Met‐Hg 2+ complex platform. We designed and fabricated methionine modified perylene diimide molecule as a selective sensing probe for Hg 2+ ions in aqueous solutions (PDI/Met‐Hg 2+ ). The complex between perylene bisimide derivative (PDI/Met) and Hg 2+ was investigated and it demonstrated turn‐on fluorescence response for the detection of the biological thiols. Besides, PDI/Met displayed fluorescence quenching response in the presence of mercury ions and the emission intensity of PDI/Met‐Hg 2+ was recovered after transferring biothiols (Cys, Hcy, and GSH). Thus, PDI/Met could be utilized as a fluorescent chemosensor for the sequential recognition of mercury ions and biological thiols. Abstract :