Multienzyme detection and in‐situ monitoring of enzyme activity by bending CE using quantum dots‐based polypeptide substrate. Issue 12 (3rd March 2020)

• Record Type: Journal Article
• Authors: Qiu, Lin; Cui, Pengfei; Zhu, Zhilan; Xu, Mingyu; Jia, Wenjing; Sheng, Jingyu; Ni, Xinye; Zhou, Shuwen; Wang, Jianhao
• Is Part Of: Electrophoresis. Volume 41:Issue 12(2020)
• Page Start: 1103
• Page End: 1108
• Publication Date: 2020-03-03
• Subjects: CE -- enzyme -- Förster resonance energy transfer -- peptide -- quantum dots; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.202000023
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract: Multienzyme detection and monitoring enzyme activity in situ are significant for the disease to diagnose. This study aims to develop a quantum dots (QDs)‐based nanoprobe Cyanine5‐DDDLEVLFQFPGLVPRGSGGHHHHHH‐QDs (Cy5‐LEVLVP‐QD), which is able to detect two enzymes inside a bent capillary using CE. Cy5‐LEVLVP and QDs were allowed to bind with each other through metal affinity interaction and then injected the Cy5‐LEVLVP‐QD complex into a capillary with different bends, followed by related enzyme that can cleave the Cy5‐LEVLVP peptide. The fluorescence of Cy5 was excited by QDs due to Förster resonance energy transfer. By monitoring the peaks produced by the original Cy5‐LEVLVP‐QD complex and a significant fluorescence change, sensitive analysis of two different enzymes was conducted. Therefore, the novel approach of using capillaries with semicircular bends could prove particularly useful for enzyme investigating in disease.