Effect of vitamin E on spermatophore regeneration and quality of pond‐reared, black tiger shrimp (Penaeus monodon). (18th February 2020)

• Record Type: Journal Article
• Authors: Jiang, Song; Liu, Dongliang; Zhou, Falin; Mo, Xianbin; Yang, Qibin; Huang, Jianhua; Yang, Lishi; Jiang, Shigui
• Is Part Of: Aquaculture research. Volume 51:Number 6(2020)
• Page Start: 2197
• Page End: 2204
• Publication Date: 2020-02-18
• Subjects: Penaeus monodon -- sperm -- spermatophore -- vitamin E; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.14524
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: The objective of the present work was to evaluate the effects of different levels of vitamin E in feed on the spermatophore regeneration and quality of male Penaeus monodon . The experiment was carried out with the four following treatments: the basal diet no added vitamin E, the diet added 200, 600 and 1, 000 mg/kg respectively. Spermatophore regeneration and quality were evaluated by spermatophore weight, sperm count and spermatophore absence rates, which male P. monodon were extruded spermatophore for feeding 20 and 40 days. In the experiment, the weight of the twice regenerated spermatophore of the males added to the vitamin E group was higher than that of the untreated control group. The weight of the first regenerated spermatophore with the addition of 1, 000 mg/kg group was the highest and significantly higher than the control group ( p < .05), but there was no significant difference among the three groups with different levels of vitamin E. The weight of the second regenerated spermatophore with the addition of 600 mg/kg group was the highest, followed by 1, 000 mg/kg group, both of which were higher than the control group and the addition of 200 mg/kg group. Within the same group, the regeneration spermatophore weight showed overall upward trend as the feeding time, twice regenerate experiment spermatophore weight with added to the vitamin E groups were significantly higher than the initial value ( p < .05), but three spermatophore weight of male shrimp at the control group had no significant difference. The sperm quantity and the percentage of normal sperm of the twice regenerated spermatophore of the males with added to the vitamin E group was higher than that of the untreated control group, and those of the addition of 200 mg/kg group was significantly higher than that of control group ( p < .05). The total number of sperm and the percentage of living sperm of male shrimp in the experimental group decreased with the increase of vitamin E in the feed. Within the same group, the total number of sperm and the percentage of living sperm of male shrimp with added to the vitamin E groups showed overall upward trend as the feeding time and were significantly higher than the initial value ( p < .05), but the control group was slightly down and had no significant difference. Comprehensive sperm weight, sperm quantity and living sperm percentage of three indicators, that adding 200 mg/kg of vitamin E in feed could effectively promote the spermatophore regeneration in the male P. monodon and improve the sperm quantity. The experimental results provide a scientific basis for the breeding of P. monodon .