Development of an integrated process for the production of high‐purity cadaverine from lysine decarboxylase. Issue 5 (17th February 2020)

• Record Type: Journal Article
• Authors: Liu, Yuxuan; Zheng, Yunxin; Wu, Hao; Zhang, Wei; Ren, Tianyu; You, Shengping; Qi, Wei; Su, Rongxin; He, Zhimin
• Is Part Of: Journal of chemical technology & biotechnology. Volume 95:Issue 5(2020)
• Page Start: 1542
• Page End: 1549
• Publication Date: 2020-02-17
• Subjects: integrated process -- l‐lysine hydrochloride -- cadaverine -- deprotonation -- n‐butanol; Biotechnology -- Periodicals; Chemistry, Technical -- Periodicals; Chemical engineering -- Periodicals; Industries -- Environmental aspects -- Periodicals; 660
• DOI: 10.1002/jctb.6348
• Languages: English
• ISSNs: 0268-2575

Abstract

Abstract: BACKGROUND: Cadaverine is a natural polyamine that can replace fossil‐derived hexamethylene diamine to produce bio‐based polyamide. Despite huge developments in cadaverine bioproduction, an econo"mically attractive purification process is a highly important part of the production of high‐purity cadaverine. RESULTS: This study utilized l ‐lysine hydrochloride as a substrate for the production of high‐purity cadaverine via a litre‐scale integrated process incorporating fermentation, bioproduction, deprotonation, extraction and rectification. First, 8.24 U mg –1 lysine decarboxylase activity and 205 g L –1 cadaverine with a yield of 91.61% were achieved through the fermentation of low‐cost industrial culture by engineered Escherichia coli and the bioproduction of cadaverine by lysine decarboxylase. Secondly, four integrated purification methods – deprotonation‐evaporation, pH adjustment‐deprotonation‐evaporation, deprotonation‐extraction‐evaporation and deprotonation‐extraction‐rectification – were explored, and the conditions of each unit operation in the chosen method (deprotonation‐extraction‐rectification) were optimized. Then the optimized method was applied to a 2‐L purification system, resulting in 99% purity of cadaverine with a yield of 87.47%. Furthermore, the n ‐butanol recovery ratio reached 91.25%, effectively avoiding the waste of resources and environmental pollution, and thus reducing purification costs. CONCLUSION: The proposed experimental approach proves that this integrated process leads to the production of high‐purity cadaverine. In the 2‐L‐scale integrated process, 358.64 g cadaverine of 99% purity was produced at a cost of US$3.99 (US$1.11 per 100 g), which is far below its market price (US$784.71 per 100 g). This integrated process provides a potential way for the industrial‐scale production of bio‐based cadaverine. © 2020 Society of Chemical Industry