Facile green synthesis approach for the production of chromium oxide nanoparticles and their different in vitro biological activities. Issue 6 (14th March 2020)

• Record Type: Journal Article
• Authors: Iqbal, Javed; Abbasi, Banzeer Ahsan; Munir, Akhtar; Uddin, Siraj; Kanwal, Sobia; Mahmood, Tariq
• Is Part Of: Microscopy research and technique. Volume 83:Issue 6(2020)
• Page Start: 706
• Page End: 719
• Publication Date: 2020-03-14
• Subjects: anticancer -- antileishmanial -- antimicrobial -- biocompatibility -- Cr2O3NPs -- protein kinase; Electron microscopy -- Technique -- Periodicals; Microscopy -- Periodicals; Microscopy -- Technique -- Periodicals; 502.825
• DOI: 10.1002/jemt.23460
• Languages: English
• ISSNs: 1059-910X

Abstract

Abstract: Green synthesis of nanoparticles using plants has become a promising substitute for the conventional chemical synthesis methods. In the present study, our aim was to synthesize chromium oxide nanoparticles (Cr2 O3 NPs) through a facile, low‐cost, eco‐friendly route using leaf extract of Rhamnus virgata (RV). The formation of Cr2 O3 NPs was confirmed and characterized by spectroscopic profile of UV–Vis, EDX, FTIR, and XRD analyses. The UV‐visible spectroscopy has confirmed the formation of Cr2 O3 NPs by the change of color owing to surface plasmon resonance. The bioactive functional groups present in the leaf extract of RV involved in reduction and stabilization of Cr2 O3 NPs were determined by FTIR analysis. Based on XRD analysis, crystalline nature of Cr2 O3 NPs was determined. The morphological shape and elemental composition of Cr2 O3 NPs were investigated using SEM and EDX analyses, respectively. With growing applications of Cr2 O3 NPs in biological perspectives, Cr2 O3 NPs were evaluated for diverse biopotentials. Cr2 O3 NPs were further investigated for its cytotoxicity potentials against HepG2 and HUH‐7 cancer cell lines (IC50 : 39.66 and 45.87 μg/ml), respectively. Cytotoxicity potential of Cr2 O3 NPs was confirmed against promastigotes (IC50 : 33.24 μg/ml) and amastigotes (IC50 : 44.31 μg/ml) using Leishmania tropica (KMH23 ). The Cr2 O3 NPs were further evaluated for antioxidants, biostatic, alpha‐amylase, and protein kinase inhibition properties. Biocompatibility assay was investigated against human macrophages which confirmed the nontoxic nature of Cr2 O3 NPs. Overall, the synthesized Cr2 O3 NPs are biocompatible and nontoxic and proved to possess significant biopotentials. In future, different in vivo studies are needed to fully investigate the cytotoxicity and mechanism of action associated with these Cr2 O3 NPs. Abstract : Aqueous extracts of Rhamnus virgata were utilized for the synthesis, characterization, and investigation of biopotential of Cr2 O3 NPs. On the whole, Cr2 O3 NPs were found to be potential in different bio‐applications.