Impact of PDGF‐BB on cellular distribution and extracellular matrix in the healing rabbit Achilles tendon three weeks post‐operation. Issue 3 (5th February 2020)

• Record Type: Journal Article
• Authors: Meier Bürgisser, Gabriella; Evrova, Olivera; Calcagni, Maurizio; Scalera, Chiara; Giovanoli, Pietro; Buschmann, Johanna
• Is Part Of: FEBS open bio. Volume 10:Issue 3(2020)
• Page Start: 327
• Page End: 337
• Publication Date: 2020-02-05
• Subjects: alpha‐SMA -- collagen -- PDGF‐BB -- proteoglycan -- rabbit Achilles tendon; Molecular biology -- Periodicals; Cytology -- Periodicals; Life sciences -- Periodicals; Biological Science Disciplines -- Periodicals; Molecular Biology -- Periodicals; Cell Biology -- Periodicals; Cytology; Life sciences; Molecular biology; Periodicals; 572.805
• DOI: 10.1002/2211-5463.12736
• Languages: English
• ISSNs: 2211-5463

Abstract

Abstract : Current methods for tendon rupture repair suffer from two main drawbacks: insufficient strength and adhesion formation, which lead to rerupture and impaired gliding. A novel polymer tube may help to overcome these problems by allowing growth factor delivery to the wound site and adhesion reduction, and by acting as a physical barrier to the surrounding tissue. In this study, we used a bilayered DegraPol ® tube to deliver PDGF‐BB to the wound site in a full‐transection rabbit Achilles tendon model. We then performed histological and immunohistochemical analysis at 3 weeks postoperation. Sustained delivery of PDGF‐BB to the healing Achilles tendon led to a significantly more homogenous cell distribution within the healing tissue. Lower cell densities next to the implant material were determined for +PDGF‐BB samples compared to −PDGF‐BB. PDGF‐BB application increased proteoglycan content and reduced alpha‐SMA + areas, clusters of different sizes, mainly vessels. Finally, PDGF‐BB reduced collagens I and III in the extracellular matrix. The sustained delivery of PDGF‐BB via an electrospun DegraPol ® tube accelerated tendon wound healing by causing a more uniform cell distribution with higher proteoglycan content and less fibrotic tissue. Moreover, the application of this growth factor reduced collagen III and alpha‐SMA, indicating a faster and less fibrotic tendon healing. Abstract : Sustained PDGF‐BB delivery via DegraPol ® implant to a fully transected rabbit Achilles tendon leads to a more homogenous cellular distribution in the healing tendon tissue in vivo . Increased proteoglycan content and reduced collagen III and alpha‐SMA + areas confirm the positive effect of PDGF‐BB.