Deletion of maternal UHRF1 severely reduces mouse oocyte quality and causes developmental defects in preimplantation embryos. Issue 7 (17th April 2019)
- Record Type:
- Journal Article
- Title:
- Deletion of maternal UHRF1 severely reduces mouse oocyte quality and causes developmental defects in preimplantation embryos. Issue 7 (17th April 2019)
- Main Title:
- Deletion of maternal UHRF1 severely reduces mouse oocyte quality and causes developmental defects in preimplantation embryos
- Authors:
- Cao, Yumeng
Li, Mingrui
Liu, Fei
Ni, Xiaobei
Wang, Shuai
Zhang, Hao
Sui, Xuesong
Huo, Ran - Abstract:
- ABSTRACT: The ubiquitin‐like, containing PHD and RING finger domains, 1 (UHRF1) protein recognizes DNA methylation and histone modification and plays a critical role in epigenetic regulation. Recently, UHRF1 was shown to have a role in DNA methylation in oocytes and early embryos. Here, we reveal that maternal UHRF1 determines the quality of mouse oocytes. We generated oocyte‐specific Uhrf1 ‐knockout mice and found that females were sterile, and few maternal UHRF1‐null embryos developed into blastocysts. The UHRF1‐null oocytes had an increased incidence of aneuploidy and DNA damage. In addition to defective DNA methylation, histone modification was affected during oogenesis, with UHRF1‐null germinal vesicle and metaphase II‐stage oocytes exhibiting reduced global histone H3 lysine 9 dimethylation levels and elevated acetylation of histone H4 lysine 12. Taken together, our results suggest that UHRF1 plays an important role in determining oocyte quality and affects epigenetic regulation of oocyte maturation as a maternal protein, which is crucial for embryo developmental potential. Further exploration of the biologic function and underlying mechanisms of maternal UHRF1 will enhance our understanding of the maternal control of the oocyte and early embryonic development.—Cao, Y., Li, M., Liu, F., Ni, X., Wang, S., Zhang, H., Sui, X., Huo, R. Deletion of maternal UHRF1 severely reduces mouse oocyte quality and causes developmental defects in preimplantation embryos. FASEB J. 33,ABSTRACT: The ubiquitin‐like, containing PHD and RING finger domains, 1 (UHRF1) protein recognizes DNA methylation and histone modification and plays a critical role in epigenetic regulation. Recently, UHRF1 was shown to have a role in DNA methylation in oocytes and early embryos. Here, we reveal that maternal UHRF1 determines the quality of mouse oocytes. We generated oocyte‐specific Uhrf1 ‐knockout mice and found that females were sterile, and few maternal UHRF1‐null embryos developed into blastocysts. The UHRF1‐null oocytes had an increased incidence of aneuploidy and DNA damage. In addition to defective DNA methylation, histone modification was affected during oogenesis, with UHRF1‐null germinal vesicle and metaphase II‐stage oocytes exhibiting reduced global histone H3 lysine 9 dimethylation levels and elevated acetylation of histone H4 lysine 12. Taken together, our results suggest that UHRF1 plays an important role in determining oocyte quality and affects epigenetic regulation of oocyte maturation as a maternal protein, which is crucial for embryo developmental potential. Further exploration of the biologic function and underlying mechanisms of maternal UHRF1 will enhance our understanding of the maternal control of the oocyte and early embryonic development.—Cao, Y., Li, M., Liu, F., Ni, X., Wang, S., Zhang, H., Sui, X., Huo, R. Deletion of maternal UHRF1 severely reduces mouse oocyte quality and causes developmental defects in preimplantation embryos. FASEB J. 33, 8294–8305 (2019). www.fasebj.org … (more)
- Is Part Of:
- FASEB journal. Volume 33:Issue 7(2019)
- Journal:
- FASEB journal
- Issue:
- Volume 33:Issue 7(2019)
- Issue Display:
- Volume 33, Issue 7 (2019)
- Year:
- 2019
- Volume:
- 33
- Issue:
- 7
- Issue Sort Value:
- 2019-0033-0007-0000
- Page Start:
- 8294
- Page End:
- 8305
- Publication Date:
- 2019-04-17
- Subjects:
- infertility -- aneuploidy -- epigenetic modification -- DNA damage
Biology -- Periodicals
Biology, Experimental -- Periodicals
570 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1096/fj.201801696RRRR ↗
- Languages:
- English
- ISSNs:
- 0892-6638
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13230.xml