Mutation of doublesex in Hyphantria cunea results in sex‐specific sterility. Issue 5 (25th December 2019)

• Record Type: Journal Article
• Authors: Li, Xiaowei; Liu, Qun; Liu, Huihui; Bi, Honglun; Wang, Yaohui; Chen, Xien; Wu, Ningning; Xu, Jun; Zhang, Zhen; Huang, Yongping; Chen, Hui
• Is Part Of: Pest management science. Volume 76:Issue 5(2020)
• Page Start: 1673
• Page End: 1682
• Publication Date: 2019-12-25
• Subjects: Hyphantria cunea -- doubelsex -- CRISPR/Cas9 system -- sex‐specific sterility -- pest management; Pests -- Control -- Periodicals; Pesticides -- Periodicals; 632.9
• DOI: 10.1002/ps.5687
• Languages: English
• ISSNs: 1526-498X

Abstract

Abstract: BACKGROUND: The gene doublesex ( dsx ) plays pivotal roles in sex determination and controls sexually dimorphic development in certain insects. Importantly, it also displays a potential candidate target for pest management due to its sex‐specific splicing. Therefore, we used CRISPR/Cas9‐mediated gene disruption to investigate the function of dsx in Hyphantria cunea, an invasive forest pest. RESULT: In the present study, we identified the dsx gene from H. cunea which showed a sex‐biased expression pattern that was different from other lepidopteran insects. Referring to sex‐specific functional analyses in Bombyx mori, we performed a site‐specific knockout of the Hcdsx gene by using a CRISPR/Cas9 system, which induced severe abnormalities in external genitalia and some incomplete sex reversal phenotypes, which in turn led to reduced sex‐specific fecundity. An alternative splicing pattern of Hcdsx was altered by CRISPR/Cas9‐induced mutation, and alterations in splicing affected expression of downstream genes encoding pheromone binding protein 1, vg1 and vg2 (encoding vitellogenin), which contributed to the sex‐specific sterility phenotypes in the Hcdsx mutants. CONCLUSION: The Hcdsx gene plays important roles in sexual differentiation in H. cunea . Disruption of Hcdsx induced sex‐specific sterility, demonstrating a potential application in control of this pest. © 2019 Society of Chemical Industry Abstract : Disruption of putative sex‐specific doublesex exons and common exon by the CRISPR/Cas9 system resulted in sex‐specific abnormal external genitals, which caused the mutants to fail to mate and produce offspring, leading to sex‐specific sterility.