Intrauterine growth restriction disrupts developmental epigenetics around distal growth hormone response elements on the rat hepatic IGF‐1 gene. Issue 4 (2nd December 2014)
- Record Type:
- Journal Article
- Title:
- Intrauterine growth restriction disrupts developmental epigenetics around distal growth hormone response elements on the rat hepatic IGF‐1 gene. Issue 4 (2nd December 2014)
- Main Title:
- Intrauterine growth restriction disrupts developmental epigenetics around distal growth hormone response elements on the rat hepatic IGF‐1 gene
- Authors:
- Fu, Qi
McKnight, Robert A.
Callaway, Christopher W.
Yu, Xing
Lane, Robert H.
Majnik, Amber V. - Abstract:
- Abstract : Intrauterine growth restriction (IUGR) decreases serum IGF‐1 levels. Postnatal IGF‐1 expression is transcriptionally regulated by growth hormone (GH) through growth hormone response elements (GHREs). We hypothesized that IUGR disrupts the normal developmental maturation of hepatic IGF‐1 intron 2 growth hormone response element (IN2GHRE) histone methylation of key lysines and DNA methylation. We also evaluated a 5' distal weak enhancer (IGF‐15'‐upstream region growth hormone response element; 5URGHRE) as a GHRE specificity control. IUGR was induced through a well‐characterized model of bilateral uterine artery ligation of the pregnant rat. Offspring livers were tested at d 0 and 21. Chromatin immunoprecipitation and bisulfite sequencing quantified epigenetic characteristics. We found that distinct age‐related developmental patterns of histone and DNA methylation characterize each GHRE. Development increased H3K4 trimethylation (me3) in both GHREs. However, H3K9me3 decreased with age at IN2GHRE and increased with age at 5URGHRE. IUGR altered the developmental pattern of H3K4me3 and K9me3 around the GHREs in a sex‐specific manner at d 21. Developmental and IUGR‐induced DNA methylation occurred in a GHRE‐, CpG site‐, and sex‐specific manner. We conclude that IUGR disrupts developmental epigenetics around distal GHREs on the rat hepatic IGF‐1 gene.—Fu, Q., McKnight, R. A., Callaway, C. W., Yu, X., Lane, R. H., Majnik, A. V. Intrauterine growth restriction disruptsAbstract : Intrauterine growth restriction (IUGR) decreases serum IGF‐1 levels. Postnatal IGF‐1 expression is transcriptionally regulated by growth hormone (GH) through growth hormone response elements (GHREs). We hypothesized that IUGR disrupts the normal developmental maturation of hepatic IGF‐1 intron 2 growth hormone response element (IN2GHRE) histone methylation of key lysines and DNA methylation. We also evaluated a 5' distal weak enhancer (IGF‐15'‐upstream region growth hormone response element; 5URGHRE) as a GHRE specificity control. IUGR was induced through a well‐characterized model of bilateral uterine artery ligation of the pregnant rat. Offspring livers were tested at d 0 and 21. Chromatin immunoprecipitation and bisulfite sequencing quantified epigenetic characteristics. We found that distinct age‐related developmental patterns of histone and DNA methylation characterize each GHRE. Development increased H3K4 trimethylation (me3) in both GHREs. However, H3K9me3 decreased with age at IN2GHRE and increased with age at 5URGHRE. IUGR altered the developmental pattern of H3K4me3 and K9me3 around the GHREs in a sex‐specific manner at d 21. Developmental and IUGR‐induced DNA methylation occurred in a GHRE‐, CpG site‐, and sex‐specific manner. We conclude that IUGR disrupts developmental epigenetics around distal GHREs on the rat hepatic IGF‐1 gene.—Fu, Q., McKnight, R. A., Callaway, C. W., Yu, X., Lane, R. H., Majnik, A. V. Intrauterine growth restriction disrupts developmental epigenetics around distal growth hormone response elements on the rat hepatic IGF‐1 gene. FASEB J. 29, 1176‐1184 (2015). www.fasebj.org … (more)
- Is Part Of:
- FASEB journal. Volume 29:Issue 4(2015)
- Journal:
- FASEB journal
- Issue:
- Volume 29:Issue 4(2015)
- Issue Display:
- Volume 29, Issue 4 (2015)
- Year:
- 2015
- Volume:
- 29
- Issue:
- 4
- Issue Sort Value:
- 2015-0029-0004-0000
- Page Start:
- 1176
- Page End:
- 1184
- Publication Date:
- 2014-12-02
- Subjects:
- prenatal -- enhancer -- methylation -- histone · DNA
Biology -- Periodicals
Biology, Experimental -- Periodicals
570 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1096/fj.14-258442 ↗
- Languages:
- English
- ISSNs:
- 0892-6638
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13224.xml