Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer. Issue 3 (6th December 2018)
- Record Type:
- Journal Article
- Title:
- Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer. Issue 3 (6th December 2018)
- Main Title:
- Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer
- Authors:
- Kang, Minsoo
Kim, Suhyun
Ko, Jesang - Abstract:
- ABSTRACT: Extracellular vesicles contain various cellular components that are involved in tumor growth, metastasis, and immune escape. Extracellular vesicles are classified into 2 groups, namely, exosomes and microvesicles (MV). Although the formation and roles of exosomes have been studied, the exact functions of MVs and mechanisms underlying MV release are not fully understood. We found that epidermal growth factor accelerates the release of MVs from the plasma membrane by inducing NF‐κB activation and CD133 expression. The amount and sizes of budding MVs were found to be dependent on the expression level of CD133, which regulates the activities of the small guanosine 5′‐triphosphatases RhoA and Rac1. CD133‐containing MVs released from KRAS mutant colon cancer cells delivered KRAS mutant to adjacent nontumorigenic cells and activated KRAS downstream signaling. CD133‐containing MVs were found to promote the migration and invasion of adjacent cells. CD133‐containing MVs induced the development of chemoresistance by abolishing the inhibitory effects of anti‐epidermal growth factor receptor (EGFR) drugs on cell proliferation and motility in colon cancer. These results suggest that CD133 acts as a novel modulator in MV release and in oncoprotein trafficking. CD133 can serve as a therapeutic target for treatment of anti‐EGFR drug‐resistant colon cancer.—Kang, M., Kim, S., Ko, J. Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer. FASEB J. 33,ABSTRACT: Extracellular vesicles contain various cellular components that are involved in tumor growth, metastasis, and immune escape. Extracellular vesicles are classified into 2 groups, namely, exosomes and microvesicles (MV). Although the formation and roles of exosomes have been studied, the exact functions of MVs and mechanisms underlying MV release are not fully understood. We found that epidermal growth factor accelerates the release of MVs from the plasma membrane by inducing NF‐κB activation and CD133 expression. The amount and sizes of budding MVs were found to be dependent on the expression level of CD133, which regulates the activities of the small guanosine 5′‐triphosphatases RhoA and Rac1. CD133‐containing MVs released from KRAS mutant colon cancer cells delivered KRAS mutant to adjacent nontumorigenic cells and activated KRAS downstream signaling. CD133‐containing MVs were found to promote the migration and invasion of adjacent cells. CD133‐containing MVs induced the development of chemoresistance by abolishing the inhibitory effects of anti‐epidermal growth factor receptor (EGFR) drugs on cell proliferation and motility in colon cancer. These results suggest that CD133 acts as a novel modulator in MV release and in oncoprotein trafficking. CD133 can serve as a therapeutic target for treatment of anti‐EGFR drug‐resistant colon cancer.—Kang, M., Kim, S., Ko, J. Roles of CD133 in microvesicle formation and oncoprotein trafficking in colon cancer. FASEB J. 33, 4248–4260 (2019). www.fasebj.org … (more)
- Is Part Of:
- FASEB journal. Volume 33:Issue 3(2019)
- Journal:
- FASEB journal
- Issue:
- Volume 33:Issue 3(2019)
- Issue Display:
- Volume 33, Issue 3 (2019)
- Year:
- 2019
- Volume:
- 33
- Issue:
- 3
- Issue Sort Value:
- 2019-0033-0003-0000
- Page Start:
- 4248
- Page End:
- 4260
- Publication Date:
- 2018-12-06
- Subjects:
- extracellular vesicles -- KRAS mutant -- chemoresistance
Biology -- Periodicals
Biology, Experimental -- Periodicals
570 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1096/fj.201802018R ↗
- Languages:
- English
- ISSNs:
- 0892-6638
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13226.xml