Role of telomeric repeat–containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells. Issue 11 (1st August 2017)
- Record Type:
- Journal Article
- Title:
- Role of telomeric repeat–containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells. Issue 11 (1st August 2017)
- Main Title:
- Role of telomeric repeat–containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells
- Authors:
- Zeng, Sicong
Liu, Lvjun
Sun, Yi
Lu, Guangxiu
Lin, Ge - Abstract:
- Abstract : This study aimed to explore the role of telomeric repeat–containing RNA (TERRA) in telomeric chromatin remodeling during the early expansion of human embryonic stem cells (hESCs). During the derivation of hESCs, histone demethylation in the telomeric region facilitates telomerase‐mediated telomere elongation. An adequate telomere repeat is essential for hESCs to acquire and/or maintain the unlimited symmetric division, which suggests that there is a link between pluripotency and telomere maintenance. The present study found that the gradual decrease in TERRA levels and related TERRA foci were correlated with telomeric length elongation in the early expansion of hESCs. In addition, TERRA participated in telomeric chromatin remodeling by cooperating with SUV39H1 (suppressor of variegation 3‐9 homolog 1/2) to propagate telomeric heterochromatin marker, histone H3 trimethylation of lysine 9. Moreover, the fibroblast growth factor signaling pathway, which is activated in hESCs, could suppress TERRA levels via telomeric repeat factor 1, which results in reduced SUV39H1 recruitment by TERRA at the telomere. Taken together, these results highlight the role of TERRA in hESC telomere elongation and homeostasis in the acquisition and/or maintenance of stem cell pluripotency.—Zeng, S., Liu, L., Sun, Y., Lu, G., Lin, G. Role of telomeric repeat–containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells. FASEB J. 31, 4783‐795Abstract : This study aimed to explore the role of telomeric repeat–containing RNA (TERRA) in telomeric chromatin remodeling during the early expansion of human embryonic stem cells (hESCs). During the derivation of hESCs, histone demethylation in the telomeric region facilitates telomerase‐mediated telomere elongation. An adequate telomere repeat is essential for hESCs to acquire and/or maintain the unlimited symmetric division, which suggests that there is a link between pluripotency and telomere maintenance. The present study found that the gradual decrease in TERRA levels and related TERRA foci were correlated with telomeric length elongation in the early expansion of hESCs. In addition, TERRA participated in telomeric chromatin remodeling by cooperating with SUV39H1 (suppressor of variegation 3‐9 homolog 1/2) to propagate telomeric heterochromatin marker, histone H3 trimethylation of lysine 9. Moreover, the fibroblast growth factor signaling pathway, which is activated in hESCs, could suppress TERRA levels via telomeric repeat factor 1, which results in reduced SUV39H1 recruitment by TERRA at the telomere. Taken together, these results highlight the role of TERRA in hESC telomere elongation and homeostasis in the acquisition and/or maintenance of stem cell pluripotency.—Zeng, S., Liu, L., Sun, Y., Lu, G., Lin, G. Role of telomeric repeat–containing RNA in telomeric chromatin remodeling during the early expansion of human embryonic stem cells. FASEB J. 31, 4783‐795 (2017). www.fasebj.org … (more)
- Is Part Of:
- FASEB journal. Volume 31:Issue 11(2017)
- Journal:
- FASEB journal
- Issue:
- Volume 31:Issue 11(2017)
- Issue Display:
- Volume 31, Issue 11 (2017)
- Year:
- 2017
- Volume:
- 31
- Issue:
- 11
- Issue Sort Value:
- 2017-0031-0011-0000
- Page Start:
- 4783
- Page End:
- 4795
- Publication Date:
- 2017-08-01
- Subjects:
- histone demethylation -- telomere length -- TERRA -- TRF1
Biology -- Periodicals
Biology, Experimental -- Periodicals
570 - Journal URLs:
- http://onlinelibrary.wiley.com/ ↗
- DOI:
- 10.1096/fj.201600939RR ↗
- Languages:
- English
- ISSNs:
- 0892-6638
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - BLDSS-3PM
British Library HMNTS - ELD Digital store - Ingest File:
- 13225.xml