Distribution of dendritic cells in the septate uterus: An immunological perspective. (10th April 2020)

• Record Type: Journal Article
• Authors: Negishi, Yasuyuki; Kato, Masahiko; Ono, Shuichi; Kuwabara, Yoshimitsu; Morita, Rimpei; Takahashi, Hidemi; Takeshita, Toshiyuki
• Is Part Of: American journal of reproductive immunology. Volume 83:Number 6(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-04-10
• Subjects: chemokine receptors -- chemokines -- dendritic cells -- septate uterus; Human reproduction -- Immunological aspects -- Periodicals; 616.69206
• DOI: 10.1111/aji.13241
• Languages: English
• ISSNs: 1046-7408

Abstract

Abstract: Problem: Septate uterus is associated with spontaneous abortion. Surgical intervention of the uterine septa (US) is frequently performed following spontaneous abortion; however, immunological mechanisms for spontaneous abortion in patients with septate uterus remain completely unknown. Method of study: A total of 12 women with septate uterus who underwent hysteroscopic metroplasty and 10 women with uterine leiomyoma who underwent total hysterectomy were enrolled as the experimental and control groups, respectively. Immune cells, dendritic cells (DCs), macrophages, T cells, natural killer cells, invariant natural killer cells, and chemokine receptors in US and uterine myometrium tissue (UMT) were analyzed using flow cytometry and immunohistochemical staining. Additionally, the chemokine production of macrophage inflammatory protein 1 alpha (MIP‐1α), regulated upon activation normal T‐cell express sequence (RANTES), and macrophage inflammatory protein 3 beta (MIP‐3β) from the viable cells obtained from the US and UMT samples was evaluated in an ex vivo study. Results: The percentage of CD141 + DCs in US was significantly lower than that in UMT. Both US and UMT showed CCR1 and CCR5 expression on CD141 + DCs; however, the production of chemokines, MIP‐1α, RANTES, and MIP‐3β was abundant in UMT‐obtained viable cells. Conclusion: The accumulation of CD141 + DCs was lower in US than that in UMT. This phenomenon may be caused by low chemokine productions in US. Our findings support the benefit of surgical intervention for septate uterus—that is, the elimination of inappropriate implantation sites.