Neuronal branching of sensory neurons is associated with BDNF‐positive eosinophils in atopic dermatitis. Issue 5 (4th February 2020)

• Record Type: Journal Article
• Authors: Guseva, Daria; Rüdrich, Urda; Kotnik, Nika; Gehring, Manuela; Patsinakidis, Nikolaos; Agelopoulos, Konstantin; Ständer, Sonja; Homey, Bernhard; Kapp, Alexander; Gibbs, Bernhard F.; Ponimaskin, Evgeni; Raap, Ulrike
• Is Part Of: Clinical & experimental allergy. Volume 50:Issue 5(2020)
• Page Start: 577
• Page End: 584
• Publication Date: 2020-02-04
• Subjects: atopic dermatitis -- BDNF -- DRG neurons -- eosinophils; Allergy -- Periodicals; Immunology -- Periodicals; 616.97
• DOI: 10.1111/cea.13560
• Languages: English
• ISSNs: 0954-7894

Abstract

Abstract: Background: Pruritus is a major symptom of atopic dermatitis (AD) and is transmitted by a subpopulation of non‐myelinated C‐type free nerve endings in the epidermis and upper dermis. Stimulation of these nerve terminals is affected by histamine, neurotrophins and physical factors. Eosinophils of patients with AD are a source of neurotrophins, including brain‐derived neurotrophic factor (BDNF), levels of which correlate with disease severity. Objective: The purpose of this study was to determine the anatomical localization of eosinophils in the skin of patients with AD with regard to peripheral nerves and to investigate whether eosinophils induce sprouting and neurite outgrowth in murine sensory neurons. Methods: Cryosections of skin derived from AD and control (NA) patients were subjected to immunofluorescence analysis with markers for eosinophils, BDNF and neuronal cells. Stimulated eosinophil supernatants were used for the treatment of cultured peripheral mouse dorsal root ganglia (DRG) neurons followed by morphometric analysis. Results: Dermal axon density and the proximity of eosinophils to nerve fibres were significantly higher in AD patients vs NA. Both neuronal projections and eosinophils expressed BDNF. Furthermore, activated eosinophil supernatants induced BDNF‐dependent mouse DRG neuron branching. Conclusions and Clinical Relevance: Our results indicate that BDNF‐positive eosinophils are also localized in close proximity with nerve fibres in AD, suggesting a functional relationship between BDNF‐expressing eosinophils and neuronal projections. These observations suggest that eosinophils may have considerable impact on pruritus by supporting sensory nerve branching.