Genome‐wide analysis and phosphorylation sites identification of the 14‐3‐3 gene family and functional characterization of MeGRF3 in cassava. Issue 2 (12th March 2020) - Chang, Lili
Abstract : The biological functionality of many members of the 14‐3‐3 gene family is regulated via phosphorylation at multiple amino acid residues. The specific phosphorylation‐mediated regulation of these proteins during cassava root tuberization, however, is not well understood. In this study, 15 different 14‐3‐3 genes (designated MeGRF1 – 15 ) were identified within the cassava genome. Based upon evolutionary conservation and structural analyses, these cassava 14‐3‐3 proteins were grouped into ε and non‐ε clusters. We found these 15 MeGRF genes to be unevenly distributed across the eight cassava chromosomes. When comparing the expression of these genes during different developmental stages, we found that three of these genes ( MeGRF9, 12 and 15 ) were overexpressed at all developmental stages at 75, 104, 135, 182 and 267 days post‐planting relative to the fibrous root stage, whereas two ( MeGRF5 and 7 ) were downregulated during these same points. In addition, the expression of most MeGRF genes changed significantly in the early and middle stages of root tuberization. This suggests that these different MeGRF genes likely play distinct regulatory roles during cassava root tuberization. Subsequently, 18 phosphorylated amino acid residues were detected on nine of these MeGRF proteins. A phosphomimetic mutation at serine‐65 in MeGRF3 in Arabidopsis thaliana (Arabidopsis) slightly influenced starch metabolism in these plants, and significantly affected the role of MeGRF3 in salt stress responses. Together these results indicate that 14‐3‐3 genes play key roles in responses to abiotic stress and the regulation of starch metabolism, offering valuable insights into the functions of these genes in cassava.