New insights into bsr‐d1‐mediated broad‐spectrum resistance to rice blast. (12th May 2020)

• Record Type: Journal Article
• Authors: Zhu, Ziwei; Yin, Junjie; Chern, Mawsheng; Zhu, Xiaobo; Yang, Chao; He, Kaiwei; Liu, Yuchen; He, Min; Wang, Jing; Song, Li; Wang, Long; Wei, Yingjie; Wang, Jichun; Liu, Jiali; Qing, Hai; Bi, Yu; Li, Mingwu; Hu, Kun; Qi, Tuo; Hou, Qingqing; Chen, Xuewei; Li, Weitao
• Is Part Of: Molecular plant pathology. Volume 21:Number 7(2020)
• Page Start: 951
• Page End: 960
• Publication Date: 2020-05-12
• Subjects: hydrogen peroxide (H2O2) -- Magnaporthe oryzae -- peroxidase -- resistance -- rice blast -- salicylic acid -- transcriptome analysis; Plant diseases -- Molecular aspects -- Periodicals; Plant-pathogen relationships -- Molecular aspects -- Periodicals; 571.936
• DOI: 10.1111/mpp.12941
• Languages: English
• ISSNs: 1464-6722

Abstract

Abstract: bsr‐d1, an allele encoding a transcription factor identified from the rice cultivar Digu, confers durable, broad‐spectrum resistance to infections by strains of Magnaporthe oryzae . bsr‐d1 was predicted to inhibit M. oryzae ‐induced expression of Bsr‐d1 RNA and degradation of hydrogen peroxide to achieve resistance to M. oryzae . However, the global effect of biological process and molecular function on blast resistance mediated by Bsr‐d1 remains unknown. In this study, we compared transcriptomic profiling between Bsr‐d1 knockout (Bsr‐d1KO) lines and the wild type, TP309. Our study revealed that bsr‐d1 mainly regulates the redox state of plant cells, but also affects amino acid and unsaturated fatty acid metabolism. We further found that BSR‐D1 indirectly regulates salicylic acid biosynthesis, metabolism, and signal transduction downstream of the activation of H2 O2 signalling in the bsr‐d1 ‐mediated immune response. Furthermore, we identified a novel peroxidase‐encoding gene, Perox3, as a new BSR‐D1 target gene that reduces resistance to M. oryzae when overexpressed in TP309. These results provide new insights into the bsr‐d1 ‐mediated blast resistance. Abstract : BSR‐D1 mainly regulates the redox state of plant cells by directly targeting another new gene, Perox3, but indirectly regulates salicylic acid biosynthesis and signal transduction after H2 O2 signalling in the bsr‐d1 ‐mediated immune response.