Alteration of T Cell Phenotypes in HIV‐Neurotuberculosis Coinfection. Issue 3 (18th November 2018)

• Record Type: Journal Article
• Authors: Rao, Deepashri; Venkataswamy, Manjunatha M.; Vasanthapuram, Ravi; Satishchandra, P.; Desai, Anita
• Is Part Of: Cytometry. Volume 98:Issue 3(2020)
• Page Start: 270
• Page End: 281
• Publication Date: 2018-11-18
• Subjects: HIV -- tuberculosis -- naïve T cell -- memory T cell -- T cell activation; Flow cytometry -- Diagnostic use -- Periodicals; Cytodiagnosis -- Periodicals; 616.07582
• DOI: 10.1002/cyto.b.21746
• Languages: English
• ISSNs: 1552-4949

Abstract

Abstract : Background: Neurotuberculosis is one of the commonest HIV associated opportunistic infections of the central nervous system in India. HIV‐TB coinfection may lead to altered frequencies of T cells, thereby influencing the course and progression of the disease. Methods: We examined the frequencies of T cell subsets in HIV infected individuals with neurotuberculosis (HIV+nTB+) as compared to individuals with HIV associated systemic TB (HIV+sTB+), asymptomatic HIV (HIV+TB‐), non‐HIV neuro TB (HIV‐nTB+), non‐HIV systemic TB (HIV‐sTB+), and healthy controls (HIV‐TB‐). Activation and senescence profiles of CD4 and CD8 T cells and memory subsets in peripheral blood mononuclear cells were studied by flow cytometry. Results: The significant observations among the T cell subsets in HIV+nTB+ were: (1) Naïve T cells : decreased CD4 T cells compared to HIV‐sTB+ ( P = 0.005); decreased CD8 T cells compared to HIV‐nTB+ and HIV‐TB‐ ( P ≤ 0.007), (2) Memory T cells : expanded CD4 TEMRA cells compared to HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ ( P ≤ 0.003); expanded CD8 TEMRA cells compared to HIV‐nTB+ and HIV‐TB‐ ( P ≤ 0.005), (3) Activated T cells : higher CD4 T cells compared to HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ ( P ≤ 0.004); higher CD8 T cells compared to HIV + TB‐, HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ ( P ≤ 0.001), and (4) Senescent T cells : increased CD8 T cells compared to HIV‐nTB+ and HIV‐TB‐ groups ( P = 0.000). Conclusions: Increased activation compared to HIV+TB‐, HIV‐nTB+, HIV‐sTB+, and HIV‐TB‐ groups and increased senescence compared to HIV‐nTB+ and HIV‐TB‐ groups were observed in CD8 T cells in HIV+nTB+, suggesting that the frequencies of these T cell subsets are altered to a greater extent in these individuals. © 2018 International Clinical Cytometry Society