A critical role of the KCa3.1 channel in mechanical stretch‐induced proliferation of rat bone marrow‐derived mesenchymal stem cells. Issue 6 (17th February 2020)

• Record Type: Journal Article
• Authors: Jia, Xiaoling; Su, Hao; Chen, Xinlan; Huang, Yangbi; Zheng, Yufan; Ji, Pei; Gao, Chao; Gong, Xianghui; Huang, Yan; Jiang, Lin‐Hua; Fan, Yubo
• Is Part Of: Journal of cellular and molecular medicine. Volume 24:Issue 6(2020)
• Page Start: 3739
• Page End: 3744
• Publication Date: 2020-02-17
• Subjects: bone marrow‐derived mesenchymal stem cells -- cell proliferation -- KCa3.1 channel -- mechanical stretch; Cytology; Medicine; Molecular Biology; Cytologie -- Périodiques; Médecine -- Périodiques; Biologie moléculaire -- Périodiques; Cytology -- Periodicals; Medicine -- Periodicals; Molecular biology -- Periodicals; 611.01805
• DOI: 10.1111/jcmm.15014
• Languages: English
• ISSNs: 1582-1838

Abstract

Abstract: Mechanical stimulation is an important factor regulating mesenchymal stem cell (MSC) functions such as proliferation. The Ca 2+ ‐activated K + channel, KCa 3.1, is critically engaged in MSC proliferation but its role in mechanical regulation of MSC proliferation remains unknown. Here, we examined the KCa 3.1 channel expression and its role in rat bone marrow‐derived MSC (BMSC) proliferation in response to mechanical stretch. Application of mechanical stretch stimulated BMSC proliferation via promoting cell cycle progression. Such mechanical stimulation up‐regulated the KCa 3.1 channel expression and pharmacological or genetic inhibition of the KCa 3.1 channel strongly suppressed stretch‐induced increase in cell proliferation and cell cycle progression. These results support that the KCa 3.1 channel plays an important role in transducing mechanical forces to MSC proliferation. Our finding provides new mechanistic insights into how mechanical stimuli regulate MSC proliferation and also a viable bioengineering approach to improve MSC proliferation.