Palmitoylated Proteins in Plasmodium falciparum‐Infected Erythrocytes: Investigation with Click Chemistry and Metabolic Labeling. (28th April 2020)

• Record Type: Journal Article
• Authors: Kilian, Nicole; Zhang, Yongdeng; LaMonica, Lauren; Hooker, Giles; Toomre, Derek; Mamoun, Choukri Ben; Ernst, Andreas M.
• Is Part Of: BioEssays. Volume 42:Number 6(2020:Jun.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-04-28
• Subjects: click chemistry/metabolic labeling -- in‐gel fluorescence -- malaria -- microscopy -- Plasmodium falciparum -- S‐palmitoylation; Molecular biology -- Periodicals; Cytology -- Periodicals; Developmental biology -- Periodicals; 572.8
• DOI: 10.1002/bies.201900145
• Languages: English
• ISSNs: 0265-9247

Abstract

Abstract: The examination of the complex cell biology of the human malaria parasite Plasmodium falciparum usually relies on the time‐consuming generation of transgenic parasites. Here, metabolic labeling and click chemistry are employed as a fast transfection‐independent method for the microscopic examination of protein S‐ palmitoylation, an important post‐translational modification during the asexual intraerythrocytic replication of P. falciparum . Applying various microscopy approaches such as confocal, single‐molecule switching, and electron microscopy, differences in the extent of labeling within the different asexual developmental stages of P. falciparum and the host erythrocytes over time are observed. Abstract : The complex erythrocytic schizogony of the malaria parasite Plasmodium falciparum requires a thorough investigation to understand its virulence mechanisms. Metabolic labeling and click chemistry are used as a fast transfection‐independent method to examine the behavior of post‐translationally modified S ‐palmitoylated proteins using microscopy approaches and in‐gel fluorescence analysis.