A Novel Eukaryote‐Like CRISPR Activation Tool in Bacteria: Features and Capabilities. (20th April 2020)

• Record Type: Journal Article
• Authors: Liu, Yang; Wang, Baojun
• Is Part Of: BioEssays. Volume 42:Number 6(2020:Jun.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-04-20
• Subjects: bacteria -- CRISPR activation -- dxCas9 -- synthetic biology -- σ54‐dependent promoters; Molecular biology -- Periodicals; Cytology -- Periodicals; Developmental biology -- Periodicals; 572.8
• DOI: 10.1002/bies.201900252
• Languages: English
• ISSNs: 0265-9247

Abstract

Abstract: CRISPR (clustered regularly interspaced short palindromic repeats) activation (CRISPRa) in bacteria is an attractive method for programmable gene activation. Recently, a eukaryote‐like, σ 54 ‐dependent CRISPRa system has been reported. It exhibits high dynamic ranges and permits flexible target site selection. Here, an overview of the existing strategies of CRISPRa in bacteria is presented, and the characteristics and design principles of the CRISPRa system are introduced. Possible scenarios for applying the eukaryote‐like CRISPRa system is discussed with corresponding suggestions for performance optimization and future functional expansion. The authors envision the new eukaryote‐like CRISPRa system enabling novel designs in multiplexed gene regulation and promoting research in the σ 54 ‐dependent gene regulatory networks among a variety of biotechnology relevant or disease‐associated bacterial species. Abstract : CRISPR activation (CRISPRa) in bacteria is an attractive method for programmable gene activation. The features and capabilities of a newly reported eukaryote‐like, σ 54 ‐dependent CRISPRa system is presented. The eukaryote‐like bacterial CRISPRa enables novel designs in synthetic gene regulation and promotes research in the σ 54 ‐dependent gene networks with broad applications envisioned.