Intra‐ and inter‐laboratory reproducibility of the rat blood Pig‐a gene mutation assay. (27th March 2020)

• Record Type: Journal Article
• Authors: Dertinger, Stephen D.; Avlasevich, Svetlana L.; Torous, Dorothea K.; Bemis, Jeffrey C.; Hove, Tamsanqa Tafara; O'Connell, Oliver; Martus, Hansjoerg; Elhajouji, Azeddine
• Is Part Of: Environmental and molecular mutagenesis. Volume 61:Number 5(2020)
• Page Start: 500
• Page End: 507
• Publication Date: 2020-03-27
• Subjects: flow cytometry -- genotoxicity -- mutation -- Pig‐a gene -- reproducibility; Mutagenesis -- Periodicals; Molecular genetics -- Periodicals; Mutagenèse -- Périodiques; Mutagenèse chimique -- Périodiques; Mutation -- Périodiques; Maladies de l'environnement -- Périodiques; Génétique moléculaire -- Périodiques; 576.542
• DOI: 10.1002/em.22367
• Languages: English
• ISSNs: 0893-6692

Abstract

Abstract: The in vivo Pig‐a assay is being used in safety studies to evaluate the potential of chemicals to induce somatic cell gene mutations. Ongoing work is aimed at developing an Organization for Economic Cooperation and Development (OECD) test guideline to support routine use for regulatory purposes (OECD project number 4.93). Among the requirements for OECD approval are demonstrations of assay reliability, including reproducibility within and among laboratories. Experiments reported herein address the reproducibility of the rat blood Pig‐a assay using the reference mutagens chlorambucil and melphalan. These agents were evaluated for their ability to induce Pig‐a mutant erythrocytes in three separate studies conducted across two laboratories. Each of the studies utilized a common treatment schedule: 28 consecutive days of exposure via oral gavage. Whereas one laboratory studied Crl:CD(SD) rats, the other laboratory used Wistar Han rats. One or two days after cessation of treatment blood samples were collected for mutant reticulocyte and mutant erythrocyte measurements that were accomplished with the same analytical technique whereby samples were depleted of wildtype erythrocytes via immunomagnetic separation followed by flow cytometric enumeration of mutant phenotype cells (MutaFlow®). Dunnett's test results showed similar qualitative outcomes within and between laboratories, that is, each chemical and each study demonstrated statistically significant, dose‐related increases in mutant reticulocyte and erythrocyte frequencies. Benchmark dose analysis (PROAST software) provided a means to quantitatively analyze the results, and the relatively tight, overlapping benchmark dose confidence intervals observed for each of the two chemicals indicate that within and between laboratory reproducibility of the Pig‐a assay are high, adding further support for the development of an OECD test guideline.