C(RGDfK)‐ and ZnTriMPyP‐Bound Polymeric Nanocarriers for Tumor‐Targeted Photodynamic Therapy. (30th April 2020)

• Record Type: Journal Article
• Authors: de las Heras, Elena; Boix‐Garriga, Ester; Bryden, Francesca; Agut, Montserrat; Mora, Margarita; Sagristá, M. Lluïsa; Boyle, Ross W.; Lange, Norbert; Nonell, Santi
• Other Names: Hasan Tayyaba guestEditor.; Kessel David guestEditor.; Greer Alexander guestEditor.
• Is Part Of: Photochemistry and photobiology. Volume 96:Number 3(2020)
• Page Start: 570
• Page End: 580
• Publication Date: 2020-04-30
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.13238
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: Active targeting strategies are currently being extensively investigated in order to enhance the selectivity of photodynamic therapy. The aim of the present research was to evaluate whether the external decoration of nanopolymeric carriers with targeting peptides could add more value to a photosensitizer formulation and increase antitumor therapeutic efficacy and selectivity. To this end, we assessed PLGA‐PLA‐PEG nanoparticles (NPs) covalently attached to a hydrophilic photosensitizer 5‐[4‐azidophenyl]‐10, 15, 20‐tri‐( N ‐methyl‐4‐pyridinium)porphyrinato zinc (II) trichloride (ZnTriMPyP) and also to c(RGDfK) peptides, in order to target αv β3 integrin‐expressing cells. In vitro phototoxicity investigations showed that the ZnTriMPyP‐PLGA‐PLA‐PEG‐c(RGDfK) nanosystem is effective at submicromolar concentrations, is devoid of dark toxicity, successfully targets αv β3 integrin‐expressing cells and is 10‐fold more potent than related nanosystems where the PS is occluded instead of covalently bound. Abstract : We have studied the photodynamic activity of c(RGDfK) peptide‐targeted PLGA‐PLA‐PEG nanoparticles with covalently bound photosensitizers against αv β3 integrin‐expressing cells. The in vitro results show that the ZnTriMPyP‐PLGA‐PLA‐PEG‐c(RGDfK) nanosystem is effective at submicromolar concentrations, is devoid of dark toxicity, successfully targets αv β3 integrin‐expressing cells and is 10‐fold more potent than related nanosystems where the PS is occluded instead of covalently bound.