Glycyrrhizic‐Acid‐Based Carbon Dots with High Antiviral Activity by Multisite Inhibition Mechanisms. Issue 13 (20th February 2020)

• Record Type: Journal Article
• Authors: Tong, Ting; Hu, Hongwei; Zhou, Junwei; Deng, Shuangfei; Zhang, Xiaotong; Tang, Wantao; Fang, Liurong; Xiao, Shaobo; Liang, Jiangong
• Is Part Of: Small. Volume 16:Issue 13(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2020-02-20
• Subjects: antiviral mechanisms -- carbon dots -- glycyrrhizic acid -- porcine reproductive and respiratory syndrome virus; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201906206
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract: With the gradual usage of carbon dots (CDs) in the area of antiviral research, attempts have been stepped up to develop new antiviral CDs with high biocompatibility and antiviral effects. In this study, a kind of highly biocompatible CDs (Gly‐CDs) is synthesized from active ingredient (glycyrrhizic acid) of Chinese herbal medicine by a hydrothermal method. Using the porcine reproductive and respiratory syndrome virus (PRRSV) as a model, it is found that the Gly‐CDs inhibit PRRSV proliferation by up to 5 orders of viral titers. Detailed investigations reveal that Gly‐CDs can inhibit PRRSV invasion and replication, stimulate antiviral innate immune responses, and inhibit the accumulation of intracellular reactive oxygen species (ROS) caused by PRRSV infection. Proteomics analysis demonstrates that Gly‐CDs can stimulate cells to regulate the expression of some host restriction factors, including DDX53 and NOS3, which are directly related to PRRSV proliferation. Moreover, it is found that Gly‐CDs also remarkably suppress the propagation of other viruses, such as pseudorabies virus (PRV) and porcine epidemic diarrhea virus (PEDV), suggesting the broad antiviral activity of Gly‐CDs. The integrated results demonstrate that Gly‐CDs possess extraordinary antiviral activity with multisite inhibition mechanisms, providing a promising candidate for alternative therapy for PRRSV infection. Abstract : Glycyrrhizic‐acid‐based carbon dots with excellent biocompatibility are prepared and used to inhibit porcine reproductive and respiratory syndrome virus (PRRSV) proliferation by up to 5 orders of viral titers. Further investigations reveal as‐prepared carbon dots can inhibit invasion and replication of PRRSV by stimulating antiviral innate immune responses, inhibiting the accumulation of intracellular reactive oxygen species and stimulating production of some host restriction factors.