Head‐attached live sperm cell for label‐free micro‐Raman evaluation of sperm DNA integrity: A preliminary study. (3rd January 2020)

• Record Type: Journal Article
• Authors: Huang, Zufang; Du, ShengRong; Liang, Xiaozhou; Sun, Yan; Chen, Xiwen; Lin, Juqiang; Li, Yongzeng; Chen, Guannan; Feng, Shangyuan
• Is Part Of: Journal of Raman spectroscopy. Volume 51:Number 4(2020)
• Page Start: 591
• Page End: 595
• Publication Date: 2020-01-03
• Subjects: DNA integrity -- head‐attached sperm cell -- micro‐Raman spectroscopy; Raman spectroscopy -- Periodicals; 535.846
• DOI: 10.1002/jrs.5811
• Languages: English
• ISSNs: 0377-0486

Abstract

Abstract: High‐quality spermatozoa are essential for intracytoplasmic sperm injection. However, there is still a lack of noninvasive, objective tools that provide biochemical characteristics to select high‐quality spermatozoa in clinical settings. In this work, a facile yet efficient approach was proposed, rendering the live sperm cell with its head being attached on the surface of a substrate, which allows the subsequent micro‐Raman‐based biochemical analysis and evaluation of sperm DNA integrity noninvasively and nondestructively. Moreover, our preliminary results show that Raman peaks at 1, 050 and 1, 095 cm −1, which are assigned to DNA backbone mode and whose spectral intensity ratio are suggested as a potential spectral biomarker for evaluation of sperm DNA integrity, can be clearly observed, thus implying our facile yet efficient technique for further potential DNA integrity assessment of live sperm cell. Abstract : There is still a lack of non‐invasive, objective tools that provide biochemical characteristics to select high‐quality spermatozoa in clinical settings. In this work, a facile yet efficient approach was proposed, rendering the live sperm cell with its head being attached on the surface of a substrate, which allows the subsequent micro‐Raman based biochemical analysis and evaluation of DNA integrity of live sperm noninvasively and non‐destructively.